TY - JOUR
T1 - Subgingival endotoxin and lipoteichoic acid modulate cytokine production in diabetic subjects
T2 - A Case–control Study
AU - Rangel, Thiago P.
AU - Reis, Aurelio A.
AU - Caponi, Lara
AU - Pena, Larissa C.S.
AU - Ruiz, Karina G.S.
AU - Santamaria, Mauro P.
AU - Mathias-Santamaria, Ingrid F.
AU - Casati, Marcio Z.
AU - Casarin, Renato C.V.
N1 - Publisher Copyright:
© 2020 Wiley Periodicals LLC
PY - 2021/7
Y1 - 2021/7
N2 - Background: Periodontal disease and diabetes mellitus (DM) are highly prevalent and interrelated diseases, resulting in altered host response microbiota. Thus, this study aimed to evaluate the impact of DM on local levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and their relationship with cytokines and matrix metalloproteinases’ (MMPs) profile. Methods: This case–control study included diabetic (n = 15) and non-diabetic (n = 15) subjects presenting Stage 3-4, Grade C, Periodontitis. Gingival crevicular fluid (GCF) was collected, and LPS and LTA levels were analyzed by enzyme-linked immunosorbent assay (ELISA), while IFN-γ, IL-10, IL-17, IL-1β, IL-4, MMP-2, and MMP-9 were measured by LUMINEX/MAGpix. Mann–Whitney and Spearman’s correlation tests were used to compared and to correlate variables (p < 0.05). Results: Higher levels of LTA, LPS, IL-10, IL-1β, and MMP-2 (p < 0.05) and lower levels of IL-17 were found in the DM group (p < 0.05). Non-diabetic subjects presented higher LPS, IFN-γ, IL-17, and MMP-2 levels and lower IL-10 concentration (p < 0.05). No significant correlation was seen between LPS and cytokine profile in non-diabetic. Local levels of LTA were positively correlated with IL-17 and MMP-2 and negatively with IL-10. Conclusion: LTA and LPS drove the inflammatory profile through the modulation of cytokines and MMPs in a different manner in DM and non-diabetic subjects.
AB - Background: Periodontal disease and diabetes mellitus (DM) are highly prevalent and interrelated diseases, resulting in altered host response microbiota. Thus, this study aimed to evaluate the impact of DM on local levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and their relationship with cytokines and matrix metalloproteinases’ (MMPs) profile. Methods: This case–control study included diabetic (n = 15) and non-diabetic (n = 15) subjects presenting Stage 3-4, Grade C, Periodontitis. Gingival crevicular fluid (GCF) was collected, and LPS and LTA levels were analyzed by enzyme-linked immunosorbent assay (ELISA), while IFN-γ, IL-10, IL-17, IL-1β, IL-4, MMP-2, and MMP-9 were measured by LUMINEX/MAGpix. Mann–Whitney and Spearman’s correlation tests were used to compared and to correlate variables (p < 0.05). Results: Higher levels of LTA, LPS, IL-10, IL-1β, and MMP-2 (p < 0.05) and lower levels of IL-17 were found in the DM group (p < 0.05). Non-diabetic subjects presented higher LPS, IFN-γ, IL-17, and MMP-2 levels and lower IL-10 concentration (p < 0.05). No significant correlation was seen between LPS and cytokine profile in non-diabetic. Local levels of LTA were positively correlated with IL-17 and MMP-2 and negatively with IL-10. Conclusion: LTA and LPS drove the inflammatory profile through the modulation of cytokines and MMPs in a different manner in DM and non-diabetic subjects.
KW - cytokines
KW - diabetes mellitus
KW - periodontitis
KW - virulence factors
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U2 - 10.1111/odi.13661
DO - 10.1111/odi.13661
M3 - Article
C2 - 33012042
AN - SCOPUS:85093518196
SN - 1354-523X
VL - 27
SP - 1325
EP - 1333
JO - Oral Diseases
JF - Oral Diseases
IS - 5
ER -