Subgingival endotoxin and lipoteichoic acid modulate cytokine production in diabetic subjects: A Case–control Study

Thiago P. Rangel, Aurelio A. Reis, Lara Caponi, Larissa C.S. Pena, Karina G.S. Ruiz, Mauro P. Santamaria, Ingrid F. Mathias-Santamaria, Marcio Z. Casati, Renato C.V. Casarin

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Background: Periodontal disease and diabetes mellitus (DM) are highly prevalent and interrelated diseases, resulting in altered host response microbiota. Thus, this study aimed to evaluate the impact of DM on local levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and their relationship with cytokines and matrix metalloproteinases’ (MMPs) profile. Methods: This case–control study included diabetic (n = 15) and non-diabetic (n = 15) subjects presenting Stage 3-4, Grade C, Periodontitis. Gingival crevicular fluid (GCF) was collected, and LPS and LTA levels were analyzed by enzyme-linked immunosorbent assay (ELISA), while IFN-γ, IL-10, IL-17, IL-1β, IL-4, MMP-2, and MMP-9 were measured by LUMINEX/MAGpix. Mann–Whitney and Spearman’s correlation tests were used to compared and to correlate variables (p < 0.05). Results: Higher levels of LTA, LPS, IL-10, IL-1β, and MMP-2 (p < 0.05) and lower levels of IL-17 were found in the DM group (p < 0.05). Non-diabetic subjects presented higher LPS, IFN-γ, IL-17, and MMP-2 levels and lower IL-10 concentration (p < 0.05). No significant correlation was seen between LPS and cytokine profile in non-diabetic. Local levels of LTA were positively correlated with IL-17 and MMP-2 and negatively with IL-10. Conclusion: LTA and LPS drove the inflammatory profile through the modulation of cytokines and MMPs in a different manner in DM and non-diabetic subjects.

Original languageEnglish
Pages (from-to)1325-1333
Number of pages9
JournalOral Diseases
Issue number5
StatePublished - Jul 2021

Bibliographical note

Publisher Copyright:
© 2020 Wiley Periodicals LLC


  • cytokines
  • diabetes mellitus
  • periodontitis
  • virulence factors

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry


Dive into the research topics of 'Subgingival endotoxin and lipoteichoic acid modulate cytokine production in diabetic subjects: A Case–control Study'. Together they form a unique fingerprint.

Cite this