Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Christoph Albermann, Aileen Soriano, Jiqing Jiang, Heidi Vollmer, John B. Biggins, William A. Barton, Jacob Lesniak, Dimitar B. Nikolov, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

(Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

Original languageEnglish
Pages (from-to)933-936
Number of pages4
JournalOrganic Letters
Volume5
Issue number6
DOIs
StatePublished - Mar 20 2003

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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