Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Christoph Albermann; Aileen Soriano; Jiqing Jiang; Heidi Vollmer; John B. Biggins; William A. Barton; Jacob Lesniak; Dimitar B. Nikolov; Jon S. Thorson

doi:10.1021/ol0341086

Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Christoph Albermann, Aileen Soriano, Jiqing Jiang, Heidi Vollmer, John B. Biggins, William A. Barton, Jacob Lesniak, Dimitar B. Nikolov, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

(Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

Original language	English
Pages (from-to)	933-936
Number of pages	4
Journal	Organic Letters
Volume	5
Issue number	6
DOIs	https://doi.org/10.1021/ol0341086
State	Published - Mar 20 2003

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.1021/ol0341086

Cite this

@article{2e4ec2612cc042cfac6b8a34878c73f0,

title = "Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization",

abstract = "(Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new {"}unnatural{"} natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.",

author = "Christoph Albermann and Aileen Soriano and Jiqing Jiang and Heidi Vollmer and Biggins, {John B.} and Barton, {William A.} and Jacob Lesniak and Nikolov, {Dimitar B.} and Thorson, {Jon S.}",

year = "2003",

month = mar,

day = "20",

doi = "10.1021/ol0341086",

language = "English",

volume = "5",

pages = "933--936",

number = "6",

}

TY - JOUR

T1 - Substrate specificity of NovM

T2 - Implications for novobiocin biosynthesis and glycorandomization

AU - Albermann, Christoph

AU - Soriano, Aileen

AU - Jiang, Jiqing

AU - Vollmer, Heidi

AU - Biggins, John B.

AU - Barton, William A.

AU - Lesniak, Jacob

AU - Nikolov, Dimitar B.

AU - Thorson, Jon S.

PY - 2003/3/20

Y1 - 2003/3/20

N2 - (Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

AB - (Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

UR - http://www.scopus.com/inward/record.url?scp=0038204472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038204472&partnerID=8YFLogxK

U2 - 10.1021/ol0341086

DO - 10.1021/ol0341086

M3 - Article

C2 - 12633109

AN - SCOPUS:0038204472

SN - 1523-7060

VL - 5

SP - 933

EP - 936

JO - Organic Letters

JF - Organic Letters

IS - 6

ER -

Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this