Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Christoph Albermann; Aileen Soriano; Jiqing Jiang; Heidi Vollmer; John B. Biggins; William A. Barton; Jacob Lesniak; Dimitar B. Nikolov; Jon S. Thorson

doi:10.1021/ol0341086

Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Christoph Albermann
, Aileen Soriano
, Jiqing Jiang
, Heidi Vollmer
, John B. Biggins
, William A. Barton
, Jacob Lesniak
, Dimitar B. Nikolov
, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

80 Citations (SciVal)

Abstract

(Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

Original language	English
Pages (from-to)	933-936
Number of pages	4
Journal	Organic Letters
Volume	5
Issue number	6
DOIs	https://doi.org/10.1021/ol0341086
State	Published - Mar 20 2003

Funding

Funders	Funder number
National Childhood Cancer Registry – National Cancer Institute	R01CA084374
National Childhood Cancer Registry – National Cancer Institute

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol0341086

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abstract = "(Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new {"}unnatural{"} natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.",

author = "Christoph Albermann and Aileen Soriano and Jiqing Jiang and Heidi Vollmer and Biggins, \{John B.\} and Barton, \{William A.\} and Jacob Lesniak and Nikolov, \{Dimitar B.\} and Thorson, \{Jon S.\}",

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T2 - Implications for novobiocin biosynthesis and glycorandomization

AU - Albermann, Christoph

AU - Soriano, Aileen

AU - Jiang, Jiqing

AU - Vollmer, Heidi

AU - Biggins, John B.

AU - Barton, William A.

AU - Lesniak, Jacob

AU - Nikolov, Dimitar B.

AU - Thorson, Jon S.

PY - 2003/3/20

Y1 - 2003/3/20

N2 - (Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

AB - (Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.

UR - https://www.scopus.com/pages/publications/0038204472

UR - https://www.scopus.com/pages/publications/0038204472#tab=citedBy

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EP - 936

JO - Organic Letters

JF - Organic Letters

IS - 6

ER -

Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

Abstract

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ASJC Scopus subject areas

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