Sulfiredoxin is an AP-1 target gene that is required for transformation and shows elevated expression in human skin malignancies

Qiou Wei, Hong Jiang, Connie P. Matthews, Nancy H. Colburn

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Previous studies have shown that a dominant negative form of c-Jun (TAM67) suppresses mouse skin carcinogenesis both in vitro and in vivo. The current study identifies Sulfiredoxin (Srx) as a unique target of activator protein-1 (AP-1) activation and TAM67 inhibition. Manipulation of Srx levels by ShRNA or over-expression demonstrates that Srx is critical for redox homeostasis through reducing hyperoxidized peroxiredoxins. In JB6 cells, knockdown of Srx abolishes tumor promoter-induced transformation and enhances cell sensitivity to oxidative stress. Knockdown of Srx also impairs c-Jun phosphorylation, implicating a role for Srx in the feedback regulation of AP-1 activity. Screening of patient tissues by tissue microarray reveals elevated Srx expression in several types of human skin cancers. Our study indicates that Srx is a functionally significant target of AP-1 blockade that may have value in cancer prevention or treatment.

Original languageEnglish
Pages (from-to)19738-19743
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number50
DOIs
StatePublished - Dec 16 2008

Keywords

  • Peroxiredoxin
  • Skin tumor
  • Tumor promotion

ASJC Scopus subject areas

  • General

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