[18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

Kiran Kumar Solingapuram Sai, Xiaofei Chen, Zhe Li, Caigang Zhu, Kirtikar Shukla, Tom E. Forshaw, Hanzhi Wu, Stephen A. Vance, Buddhika Liyana Pathirannahel, Megan Madonna, Mark W. Dewhirst, Allen W. Tsang, Leslie B. Poole, Nimmi Ramanujam, S. Bruce King, Cristina M. Furdui

Research output: Contribution to journalArticlepeer-review

Abstract

Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [18F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.

Original languageEnglish
Article number102218
JournalRedox Biology
Volume49
DOIs
StatePublished - Feb 2022

Bibliographical note

Funding Information:
The authors acknowledge financial support for these studies provided by the Center for Redox Biology and Medicine at Wake Forest School of Medicine (pilot funds to S.B.K.), the Wake Forest Baptist Comprehensive Cancer Center (NIH/NCI P30 CA12197 ; pilot funds to C.M.F. and support of shared resources facilities), NIH/NCI R42 CA156901 to N.R., and NIH/NIGMS R35 GM135179 to L.B.P..

Publisher Copyright:
© 2021

Keywords

  • Head and Neck Squamous Cell Cancer
  • PET imaging
  • Protein sulfenylation
  • Radiation resistance
  • [18F]F-DCP

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

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