Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

Ding Yi Hui Lu; Hao Yue; Li Ping Huang; De Yong Zhang; Zhan Hong Zhang; Zhuo Zhang; Youjun Zhang; Fan Li; Fei Yan; Xu Guo Zhou; Xiao Bin Shi; Yong Liu

doi:10.1002/ps.6572

Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

Ding Yi Hui Lu, Hao Yue, Li Ping Huang, De Yong Zhang, Zhan Hong Zhang, Zhuo Zhang, Youjun Zhang, Fan Li, Fei Yan, Xu Guo Zhou, Xiao Bin Shi, Yong Liu

Entomology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

Original language	English
Pages (from-to)	5294-5303
Number of pages	10
Journal	Pest Management Science
Volume	77
Issue number	11
DOIs	https://doi.org/10.1002/ps.6572
State	Published - Nov 2021

Bibliographical note

Funding Information:
The authors thank Prof. Tao Zhou (China Agricultural University) for providing the infectious cDNA clone of ToCV. This study was financed by the National Natural Science Foundation of China (31872932, 32030088, 31972242 and 32072383), the Hunan Natural Science Foundation (2019JJ30014), the Agriculture Research System of China (CARS‐23‐D‐02) and the National Key Research and Development Project (2018YFE0112600).

Publisher Copyright:
© 2021 Society of Chemical Industry.

Keywords

Bemisia tabaci
RNA interference
tomato chlorosis virus
α-glucosidase

ASJC Scopus subject areas

Agronomy and Crop Science
Insect Science

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10.1002/ps.6572

Cite this

@article{68035ac37158410d8500aefb06c50432,

title = "Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci",

abstract = "BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.",

keywords = "Bemisia tabaci, RNA interference, tomato chlorosis virus, α-glucosidase",

author = "Lu, {Ding Yi Hui} and Hao Yue and Huang, {Li Ping} and Zhang, {De Yong} and Zhang, {Zhan Hong} and Zhuo Zhang and Youjun Zhang and Fan Li and Fei Yan and Zhou, {Xu Guo} and Shi, {Xiao Bin} and Yong Liu",

note = "Funding Information: The authors thank Prof. Tao Zhou (China Agricultural University) for providing the infectious cDNA clone of ToCV. This study was financed by the National Natural Science Foundation of China (31872932, 32030088, 31972242 and 32072383), the Hunan Natural Science Foundation (2019JJ30014), the Agriculture Research System of China (CARS‐23‐D‐02) and the National Key Research and Development Project (2018YFE0112600). Publisher Copyright: {\textcopyright} 2021 Society of Chemical Industry.",

year = "2021",

month = nov,

doi = "10.1002/ps.6572",

language = "English",

volume = "77",

pages = "5294--5303",

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TY - JOUR

T1 - Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

AU - Lu, Ding Yi Hui

AU - Yue, Hao

AU - Huang, Li Ping

AU - Zhang, De Yong

AU - Zhang, Zhan Hong

AU - Zhang, Zhuo

AU - Zhang, Youjun

AU - Li, Fan

AU - Yan, Fei

AU - Zhou, Xu Guo

AU - Shi, Xiao Bin

AU - Liu, Yong

N1 - Funding Information: The authors thank Prof. Tao Zhou (China Agricultural University) for providing the infectious cDNA clone of ToCV. This study was financed by the National Natural Science Foundation of China (31872932, 32030088, 31972242 and 32072383), the Hunan Natural Science Foundation (2019JJ30014), the Agriculture Research System of China (CARS‐23‐D‐02) and the National Key Research and Development Project (2018YFE0112600). Publisher Copyright: © 2021 Society of Chemical Industry.

PY - 2021/11

Y1 - 2021/11

N2 - BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

AB - BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

KW - Bemisia tabaci

KW - RNA interference

KW - tomato chlorosis virus

KW - α-glucosidase

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DO - 10.1002/ps.6572

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C2 - 34310017

AN - SCOPUS:85112625001

SN - 1526-498X

VL - 77

SP - 5294

EP - 5303

JO - Pest Management Science

JF - Pest Management Science

IS - 11

ER -

Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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