Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

Ding Yi Hui Lu, Hao Yue, Li Ping Huang, De Yong Zhang, Zhan Hong Zhang, Zhuo Zhang, Youjun Zhang, Fan Li, Fei Yan, Xu Guo Zhou, Xiao Bin Shi, Yong Liu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

Original languageEnglish
Pages (from-to)5294-5303
Number of pages10
JournalPest Management Science
Issue number11
StatePublished - Nov 2021

Bibliographical note

Funding Information:
The authors thank Prof. Tao Zhou (China Agricultural University) for providing the infectious cDNA clone of ToCV. This study was financed by the National Natural Science Foundation of China (31872932, 32030088, 31972242 and 32072383), the Hunan Natural Science Foundation (2019JJ30014), the Agriculture Research System of China (CARS‐23‐D‐02) and the National Key Research and Development Project (2018YFE0112600).

Publisher Copyright:
© 2021 Society of Chemical Industry.


  • Bemisia tabaci
  • RNA interference
  • tomato chlorosis virus
  • α-glucosidase

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science


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