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Suppression of Fibrosarcoma Metastasis by Elevated Expression of Manganese Superoxide Dismutase1

  • S. E. Safford
  • , T. D. Oberley
  • , M. Urano
  • , D. K. Clair

Research output: Contribution to journalArticlepeer-review

145 Scopus citations

Abstract

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese superoxide dismutase, was transfected with a human manganese superoxide dismutase complementary DNA Fifty clones were screened for manganese superoxide dismutase activity by the superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction assay in the presence of cyanide. Three cell lines exhibiting a range of activity were chosen to be transplanted into syngeneic mice. The results indicated that the metastasis rate for all transfected cells was significantly less than that of control cells.

Original languageEnglish
Pages (from-to)4261-4265
Number of pages5
JournalCancer Research
Volume54
Issue number16
StatePublished - Aug 1994

Funding

FundersFunder number
National Childhood Cancer Registry – National Cancer InstituteR29CA049797

    UN SDGs

    This output contributes to the following UN Sustainable Development Goals (SDGs)

    1. SDG 3 - Good Health and Well-being
      SDG 3 Good Health and Well-being

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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