Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase

Weixiong Zhong, Larry W. Oberley, Terry D. Oberley, Daret K. St Clair

Research output: Contribution to journalArticlepeer-review

222 Scopus citations


Manganese superoxide dismutase (MnSOD) has been previously shown to suppress the malignant phenotype of human melanoma and breast cancer cells. To test the possible role of MnSOD in glioma malignancy, MnSOD was overexpressed in wild type human glioma U118 cells and subcloned U118-9 cells by transfection of human MnSOD cDNA. The MnSOD-transfected cell lines demonstrated expression of exogenous (plasmid) MnSOD mRNA, increase in MnSOD immunoreactive protein, and a three- to eightfold increase in MnSOD enzymatic activity. The MnSOD overexpressing cell lines became less malignant as demonstrated by requiring a higher serum concentration to grow in vitro and much slower tumor growth in nude mice than the parental and neo control cell lines. These findings further support the hypothesis that MnSOD may be a tumor suppressor gene in a wide variety of human tumors.

Original languageEnglish
Pages (from-to)481-490
Number of pages10
Issue number4
StatePublished - 1997

Bibliographical note

Funding Information:
The authors would like to thank Hong Gao and Joan Sempf for technical assistance. This work was supported by NIH grants CA 41267 and DE 10758 to LW Oberley, CA 49797 to DK St Clair and University of Wisconisn Medical School grant to TD Oberley.


  • Antioxidant enzymes
  • Gene transfection
  • Glioma
  • MnSOD
  • Reactive oxygen species
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research


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