Surface and ultrastructural characterization of raw and pretreated switchgrass

Bryon S. Donohoe; Todd B. Vinzant; Richard T. Elander; Venkata Ramesh Pallapolu; Y. Y. Lee; Rebecca J. Garlock; Venkatesh Balan; Bruce E. Dale; Youngmi Kim; Nathan S. Mosier; Michael R. Ladisch; Matthew Falls; Mark T. Holtzapple; Rocio Sierra-Ramirez; Jian Shi; Mirvat A. Ebrik; Tim Redmond; Bin Yang; Charles E. Wyman; Bonnie Hames; Steve Thomas; Ryan E. Warner

doi:10.1016/j.biortech.2011.03.092

Surface and ultrastructural characterization of raw and pretreated switchgrass

Bryon S. Donohoe, Todd B. Vinzant, Richard T. Elander, Venkata Ramesh Pallapolu, Y. Y. Lee, Rebecca J. Garlock, Venkatesh Balan, Bruce E. Dale, Youngmi Kim, Nathan S. Mosier, Michael R. Ladisch, Matthew Falls, Mark T. Holtzapple, Rocio Sierra-Ramirez, Jian Shi, Mirvat A. Ebrik, Tim Redmond, Bin Yang, Charles E. Wyman, Bonnie HamesSteve Thomas, Ryan E. Warner

Biosystems and Agricultural Engineering

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.

Original language	English
Pages (from-to)	11097-11104
Number of pages	8
Journal	Bioresource Technology
Volume	102
Issue number	24
DOIs	https://doi.org/10.1016/j.biortech.2011.03.092
State	Published - Dec 2011

Bibliographical note

Funding Information:
Funding for this work was provided by the US Department of Energy’s Office of Biomass Program. The authors would like to thank the CAFI team members and students for providing the samples and performing the pretreatment, compositional analysis, and enzymatic digestibility analysis that supported this work.

Keywords

Biomass
Enzymatic hydrolysis
Microscopy
Pretreatment
Switchgrass

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2011.03.092

Cite this

Donohoe, B. S., Vinzant, T. B., Elander, R. T., Pallapolu, V. R., Lee, Y. Y., Garlock, R. J., Balan, V., Dale, B. E., Kim, Y., Mosier, N. S., Ladisch, M. R., Falls, M., Holtzapple, M. T., Sierra-Ramirez, R., Shi, J., Ebrik, M. A., Redmond, T., Yang, B., Wyman, C. E., ... Warner, R. E. (2011). Surface and ultrastructural characterization of raw and pretreated switchgrass. Bioresource Technology, 102(24), 11097-11104. https://doi.org/10.1016/j.biortech.2011.03.092

@article{8b6ead5e0de1474d8e5a364d6747d87d,

title = "Surface and ultrastructural characterization of raw and pretreated switchgrass",

abstract = "The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.",

keywords = "Biomass, Enzymatic hydrolysis, Microscopy, Pretreatment, Switchgrass",

author = "Donohoe, {Bryon S.} and Vinzant, {Todd B.} and Elander, {Richard T.} and Pallapolu, {Venkata Ramesh} and Lee, {Y. Y.} and Garlock, {Rebecca J.} and Venkatesh Balan and Dale, {Bruce E.} and Youngmi Kim and Mosier, {Nathan S.} and Ladisch, {Michael R.} and Matthew Falls and Holtzapple, {Mark T.} and Rocio Sierra-Ramirez and Jian Shi and Ebrik, {Mirvat A.} and Tim Redmond and Bin Yang and Wyman, {Charles E.} and Bonnie Hames and Steve Thomas and Warner, {Ryan E.}",

note = "Funding Information: Funding for this work was provided by the US Department of Energy{\textquoteright}s Office of Biomass Program. The authors would like to thank the CAFI team members and students for providing the samples and performing the pretreatment, compositional analysis, and enzymatic digestibility analysis that supported this work. ",

year = "2011",

month = dec,

doi = "10.1016/j.biortech.2011.03.092",

language = "English",

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Donohoe, BS, Vinzant, TB, Elander, RT, Pallapolu, VR, Lee, YY, Garlock, RJ, Balan, V, Dale, BE, Kim, Y, Mosier, NS, Ladisch, MR, Falls, M, Holtzapple, MT, Sierra-Ramirez, R, Shi, J, Ebrik, MA, Redmond, T, Yang, B, Wyman, CE, Hames, B, Thomas, S & Warner, RE 2011, 'Surface and ultrastructural characterization of raw and pretreated switchgrass', Bioresource Technology, vol. 102, no. 24, pp. 11097-11104. https://doi.org/10.1016/j.biortech.2011.03.092

TY - JOUR

T1 - Surface and ultrastructural characterization of raw and pretreated switchgrass

AU - Donohoe, Bryon S.

AU - Vinzant, Todd B.

AU - Elander, Richard T.

AU - Pallapolu, Venkata Ramesh

AU - Lee, Y. Y.

AU - Garlock, Rebecca J.

AU - Balan, Venkatesh

AU - Dale, Bruce E.

AU - Kim, Youngmi

AU - Mosier, Nathan S.

AU - Ladisch, Michael R.

AU - Falls, Matthew

AU - Holtzapple, Mark T.

AU - Sierra-Ramirez, Rocio

AU - Shi, Jian

AU - Ebrik, Mirvat A.

AU - Redmond, Tim

AU - Yang, Bin

AU - Wyman, Charles E.

AU - Hames, Bonnie

AU - Thomas, Steve

AU - Warner, Ryan E.

N1 - Funding Information: Funding for this work was provided by the US Department of Energy’s Office of Biomass Program. The authors would like to thank the CAFI team members and students for providing the samples and performing the pretreatment, compositional analysis, and enzymatic digestibility analysis that supported this work.

PY - 2011/12

Y1 - 2011/12

N2 - The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.

AB - The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.

KW - Biomass

KW - Enzymatic hydrolysis

KW - Microscopy

KW - Pretreatment

KW - Switchgrass

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ER -

Surface and ultrastructural characterization of raw and pretreated switchgrass

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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