TY - CHAP
T1 - Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus
AU - Rajendran, K. S.
AU - Nagy, Peter D.
PY - 2008
Y1 - 2008
N2 - Replication of the viral RNA genome performed by the viral replicase is the central process during the viral infection cycle (Nagy and Pogany, see earlier chapter four). Most RNA viruses assign one or more proteins translated from their own genomes for assembling the viral replicase complex, which consists of the viral RNA, viral proteins, and several subverted host proteins embedded in cellular membranes. Understanding the various biochemical activities of the replication proteins can lead to target identification for human intervention to control viral infections or the damage to the host cells. The replicase proteins of tomato bushy stunt virus (TBSV) are selected as model system to study the dynamics of interactions between viral replicase proteins using surface plasmon resonance (SPR) analysis. The SPR assay provides real-time protein interaction data by measuring the change in refractive index at the surface of the sensor chip due to the change in mass resulting from the interaction between the immobilized protein and the protein that is being passed over the immobilized chip surface. SPR-based biosensor BIAcore X was used to carry out TBSV replicase protein interaction studies.
AB - Replication of the viral RNA genome performed by the viral replicase is the central process during the viral infection cycle (Nagy and Pogany, see earlier chapter four). Most RNA viruses assign one or more proteins translated from their own genomes for assembling the viral replicase complex, which consists of the viral RNA, viral proteins, and several subverted host proteins embedded in cellular membranes. Understanding the various biochemical activities of the replication proteins can lead to target identification for human intervention to control viral infections or the damage to the host cells. The replicase proteins of tomato bushy stunt virus (TBSV) are selected as model system to study the dynamics of interactions between viral replicase proteins using surface plasmon resonance (SPR) analysis. The SPR assay provides real-time protein interaction data by measuring the change in refractive index at the surface of the sensor chip due to the change in mass resulting from the interaction between the immobilized protein and the protein that is being passed over the immobilized chip surface. SPR-based biosensor BIAcore X was used to carry out TBSV replicase protein interaction studies.
KW - BIAcore X
KW - Protein interaction kinetics
KW - Protein-protein interaction
KW - Replicase proteins
KW - Surface plasmon resonance
KW - Tomato bushy stunt virus
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U2 - 10.1007/978-1-59745-102-4_19
DO - 10.1007/978-1-59745-102-4_19
M3 - Chapter
C2 - 18370262
AN - SCOPUS:84934440420
SN - 9781588298270
T3 - Methods in Molecular Biology
SP - 267
EP - 277
BT - Plant Virology Protocols
ER -