SURFACE TENSION-MEDIATED PROCESSING OF WASTEWATER SAMPLES FOR SARS-CoV-2

William Strike, Atena Amirsoleimani, Abisola Olaleye, Ann Noble, Kevin Lewis, Lee Faulkner, Spencer Backus, Sierra Lindeman, Katrina Eterovich, Melicity Fraley, Eli Zeitlow, Todd Brann, Shakira Hobbs, Joseph Monroe, David Hibbard, James Keck, Scott Berry

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Wastewater testing for SARS-CoV-2 has emerged as a promising tool for disease surveillance in aggregate populations. We present a novel method to rapidly extract, concentrate, and amplify viral RNA from wastewater using Exclusion-based Sample Preparation (ESP) and RT-PCR. This technology identified potential outbreaks of SARS-CoV-2 at University of Kentucky dormitories, resulting in targeted clinical testing and quarantine procedures.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages849-850
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: Oct 10 2021Oct 14 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2110/14/21

Bibliographical note

Funding Information:
This research was funded by the Centers for Disease Control and Prevention, National Institute of Drug Abuse, and National Institute of Environmental Health Sciences. Reagents were generously provided by BEI Resources

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

  • Molecular Diagnostics
  • SARS-CoV-2
  • Surface Tension

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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