Surprising production of a new urdamycin derivative by S. fradiae ΔurdQ/R

M. Fedoryshyn, M. Nur-e-Alam, L. Zhu, A. Luzhetskyy, J. Rohr, A. Bechthold

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


A strain (S. fradiae ΔurdQ/R) with mutations in urdQ and urdR encoding a dTDP-hexose-3,4-dehydratase and a dTDP-hexose-4-ketoreductase, respectively, produces a new urdamycin analogue (urdamycin X) with changes in the polyketide structure. The structure of urdamycin X has been elucidated by NMR spectroscopy. Urdamycin X was not detectable, even in small amounts, in either S. fradiae ΔurdQ, in S. fradiae ΔurdR or in S. fradiae A0, a mutant lacking all glycosyltransferase genes. Complementation of S. fradiae ΔurdQ/R restored urdamycin A production indicating that the mutations did not cause any polar effect.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalJournal of Biotechnology
Issue number1
StatePublished - May 31 2007

Bibliographical note

Funding Information:
This work was supported by the Deutsche Forschungs-gemeinschaft (DFG) grant 6-1 to A.B. and by NIH grant CA 102102 to J.R. We thank Prof. Dr. J. Salas for providing plasmids pLR234Δ7 and pUC18U.


  • Deoxysugar biosynthetic enzymes
  • Secondary metabolism
  • Streptomyces fradiae
  • Urdamycins

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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