Surveys for maternally-inherited endosymbionts reveal novel and variable infections within solitary bee species

Abiya Saeed; Jennifer A. White

doi:10.1016/j.jip.2015.09.011

Surveys for maternally-inherited endosymbionts reveal novel and variable infections within solitary bee species

Abiya Saeed, Jennifer A. White

Entomology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Maternally-inherited bacteria can affect the fitness and population dynamics of their host insects; for solitary bees, such effects have the potential to influence bee efficacy as pollinators. We screened bee species for bacterial associates using 454-pyrosequencing (4 species) and diagnostic PCR (183 specimens across 29 species). The endosymbiont Wolbachia was abundant, infecting 18 species, including all specimens from the family Halictidae. Among commercially-supplied orchard bees (family Megachilidae), only 2/7 species were Wolbachia-infected, but one species showed variable infection among specimens. Two other maternally-inherited bacteria, Arsenophonus and Sodalis, were also detected, neither of which was fixed in infection frequency. Differential endosymbiont infection could potentially compromise fitness and reproductive compatibility among commercially redistributed pollinator populations.

Original language	English
Pages (from-to)	111-114
Number of pages	4
Journal	Journal of Invertebrate Pathology
Volume	132
DOIs	https://doi.org/10.1016/j.jip.2015.09.011
State	Published - Nov 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

Funding

We thank A. Dehnel, A. Maldonado, and A. Styer for technical support, and K. Clark, E. Dobbs, D. Hunter, R. Lee, D. Shreeve, K. Strickland, and E. Sugden for assistance in acquiring specimens. In addition, we thank L. Ayres, T. Boyd, K. Evans, C. Peek, and D. Reed for the use of their orchards in bee collections. The information reported in this paper (No. 15-08-113) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This research was funded by the University of Kentucky Department of Entomology , and USDA National Institute of Food and Agriculture Hatch project KY008052. AS was supported by Kentucky’s National Science Foundation Experimental Program to Stimulate Competitive Research , grant 0814194 .

Funders	Funder number
Kentucky’s National Science Foundation	0814194
USDA National Institute of Food and Agriculture Hatch	KY008052
University of Kentucky Department of Entomology

Keywords

Arsenophonus
Bacterial endosymbionts
Hymenoptera
Sodalis
Vertical transmission
Wolbachia

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/j.jip.2015.09.011

Cite this

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title = "Surveys for maternally-inherited endosymbionts reveal novel and variable infections within solitary bee species",

abstract = "Maternally-inherited bacteria can affect the fitness and population dynamics of their host insects; for solitary bees, such effects have the potential to influence bee efficacy as pollinators. We screened bee species for bacterial associates using 454-pyrosequencing (4 species) and diagnostic PCR (183 specimens across 29 species). The endosymbiont Wolbachia was abundant, infecting 18 species, including all specimens from the family Halictidae. Among commercially-supplied orchard bees (family Megachilidae), only 2/7 species were Wolbachia-infected, but one species showed variable infection among specimens. Two other maternally-inherited bacteria, Arsenophonus and Sodalis, were also detected, neither of which was fixed in infection frequency. Differential endosymbiont infection could potentially compromise fitness and reproductive compatibility among commercially redistributed pollinator populations.",

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AB - Maternally-inherited bacteria can affect the fitness and population dynamics of their host insects; for solitary bees, such effects have the potential to influence bee efficacy as pollinators. We screened bee species for bacterial associates using 454-pyrosequencing (4 species) and diagnostic PCR (183 specimens across 29 species). The endosymbiont Wolbachia was abundant, infecting 18 species, including all specimens from the family Halictidae. Among commercially-supplied orchard bees (family Megachilidae), only 2/7 species were Wolbachia-infected, but one species showed variable infection among specimens. Two other maternally-inherited bacteria, Arsenophonus and Sodalis, were also detected, neither of which was fixed in infection frequency. Differential endosymbiont infection could potentially compromise fitness and reproductive compatibility among commercially redistributed pollinator populations.

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Surveys for maternally-inherited endosymbionts reveal novel and variable infections within solitary bee species

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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