Synaptic connections of starburst amacrine cells and localization of acetylcholine receptors in primate retinas

Elizabeth S. Yamada, Nina Dmitrieva, Kent T. Keyser, Jon M. Lindstrom, Louis B. Hersh, David W. Marshak

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Starburst amacrine cells in the macaque retina were studied by electron microscopic immunohistochemistry. We found that these amacrine cells make a type of synapse not described previously; they are presynaptic to axon terminals of bipolar cells. We also confirmed that starburst amacrine cells are presynaptic to ganglion cell dendrites and amacrine cell processes. In order to determine the functions of these synapses, we localized acetylcholine receptors using a monoclonal antibody (mAb210) that recognizes human α3- and α5-containing nicotinic receptors and also antisera against the five known subtypes of muscarinic receptors. The majority of the mAb210-immunoreactive perikarya were amacrine cells and ganglion cells, but a subpopulation of bipolar cells was also labeled. A subset of bipolar cells and a subset of horizontal cells were labeled with antibodies to M3 muscarinic receptors. A subset of amacrine cells, including those that contain cholecystokinin, were labeled with antibodies to M2 receptors. Taken together, these results suggest that acetylcholine can modulate the activity of retinal ganglion cells by multiple pathways.

Original languageEnglish
Pages (from-to)76-90
Number of pages15
JournalJournal of Comparative Neurology
Volume461
Issue number1
DOIs
StatePublished - Jun 16 2003

Keywords

  • Acetylcholine
  • Cholinergic
  • Muscarinic
  • Nicotinic
  • Presynaptic receptors

ASJC Scopus subject areas

  • General Neuroscience

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