Synaptogenesis and calcium current distribution in cultured leech neurons

F. Fernandez-de-Miguel, R. L. Cooper, W. B. Adams

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The distribution of calcium (Ca2+) currents was studied by loose-patch technique in Retzius cells isolated from the leech central nervous system. Ca2+-tail currents were recorded in patches held at the equilibrium potential for potassium (E(K)). Measurements were made from single cells and paired cells that had formed chemical or electrical synapses in culture. In single Retzius cells, Ca2+ currents in the stump of the initial process were three times larger than in the middle part of the soma; intermediate amplitudes were found in the polar region of the soma. After the formation of chemical synapses, Ca2+ currents at the stumps of postsynaptic cells and in the polar region of the somas of presynaptic cells were reduced. The formation of electrical synapses did not affect Ca2+ current distribution.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalProceedings of the Royal Society B: Biological Sciences
Issue number1320
StatePublished - 1992

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Environmental Science
  • General Agricultural and Biological Sciences


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