Synthesis and analysis of degradation, mechanical and toxicity properties of poly(β-amino ester) degradable hydrogels

Ashley M. Hawkins, Todd A. Milbrandt, David A. Puleo, J. Zach Hilt

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Biodegradable hydrogels have been studied extensively in recent years for biomedical applications, including tissue engineering and drug delivery, due to their tunable properties and similarities to natural soft tissue. In this work, two poly(β-amino ester) biodegradable hydrogel systems were synthesised and studied in vitro. Different degradation profiles were observed, ranging from 7 h to 4 months, and the compressive modulus was shown to decrease as degradation proceeded. MTT cytotoxicity analysis was used to analyze the cellular response to the degradation products, and the hydrogel systems exhibited similar toxicity to poly(dl-lactide-co-glycolide) degradation products. Finally, cell attachment was studied by seeding pluripotent mesenchymal cells directly onto the hydrogel surfaces followed by a live/dead assay and fluorescent imaging. Cells showed significant viability at 24 h (98%) and slightly lower, but still substantial viability, at 48 h (72%). These hydrogels exhibited a range of properties and favorable cellular responses, all which indicate that these materials could be viable materials for tissue regeneration and other biomedical applications.

Original languageEnglish
Pages (from-to)1956-1964
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number5
DOIs
StatePublished - May 2011

Keywords

  • Biodegradable hydrogels
  • Cell attachment
  • Cytotoxicity
  • Poly(β-amino ester)
  • Tissue regeneration

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

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