Synthesis and characterization of BODIPY-labeled colchicine

Leggy A. Arnold; Patricia Ranaivo; R. Kiplin Guy

doi:10.1016/j.bmcl.2008.07.068

Synthesis and characterization of BODIPY-labeled colchicine

Leggy A. Arnold, Patricia Ranaivo, R. Kiplin Guy

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

Original language	English
Pages (from-to)	5867-5870
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2008.07.068
State	Published - Nov 15 2008

Bibliographical note

Funding Information:
This work was supported by the American Lebanese and Syrian Associated Charities (ALSAC), and St. Jude Children’s Research Hospital. We thank Michele Connelly for culturing the cell lines used.

Keywords

Colchicine

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2008.07.068

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title = "Synthesis and characterization of BODIPY-labeled colchicine",

abstract = "Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.",

keywords = "Colchicine",

author = "Arnold, {Leggy A.} and Patricia Ranaivo and Guy, {R. Kiplin}",

note = "Funding Information: This work was supported by the American Lebanese and Syrian Associated Charities (ALSAC), and St. Jude Children{\textquoteright}s Research Hospital. We thank Michele Connelly for culturing the cell lines used. ",

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AU - Arnold, Leggy A.

AU - Ranaivo, Patricia

AU - Guy, R. Kiplin

N1 - Funding Information: This work was supported by the American Lebanese and Syrian Associated Charities (ALSAC), and St. Jude Children’s Research Hospital. We thank Michele Connelly for culturing the cell lines used.

PY - 2008/11/15

Y1 - 2008/11/15

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AB - Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 22

ER -

Synthesis and characterization of BODIPY-labeled colchicine

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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