Systemic inflammatory responses in patients with type 2 diabetes with chronic periodontitis

Ruben Mesia, Fatemeh Gholami, Hong Huang, Michael Clare-Salzler, Ikramuddin Aukhil, Shannon M. Wallet, Luciana M. Shaddox

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32 Scopus citations

Abstract

Objective: The objective of this case-control study was to quantify the immune responsiveness in individuals with type 2 diabetes (T2D) as compared with patients without diabetes (NT2D) diagnosed with periodontitis. Research Design and Methods: Peripheral blood was collected from 20 patients with moderate-to-severe chronic periodontitis (10 T2D, 10 NT2D). Blood samples were stimulated with ultrapure Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) for 24 hours. 14 cytokines/ chemokines were quantified in culture supernatants using multiplex technology. Results: T2D individuals demonstrated higher unstimulated levels of interleukin 6 (IL-6), IL-1β, tumor necrosis factor α, interferon γ, IL-10, IL-8, macrophage inflammatory protein 1α (MIP1α), and 1β (MIP1β), and higher stimulated levels of IL-6, IL-8, IL-10, MIP1α and MIP1β, along with lower unstimulated and stimulated levels of granulocyte-macrophage colonystimulating factor (GM-CSF) when compared with NT2D (p<0.05). Importantly, the LPS-induced levels of IL-6, IL-8, IL-10 and MIP1α strongly correlated with severity of disease, measured by pocket depths (PD), within the T2D group (r2≥0.7, p<0.05), but not within NT2D. Conclusions: Among patients with chronic periodontitis, patients with T2D seem to have an enhanced LPS-induced immune responsiveness than individuals without diabetes, which correlates with periodontal disease severity, concomitant with a less robust GM-CSF response. This data may in part explain the higher predisposition to periodontitis in this population.

Original languageEnglish
Article numbere000260
JournalBMJ Open Diabetes Research and Care
Volume4
Issue number1
DOIs
StatePublished - Sep 1 2016

Bibliographical note

Publisher Copyright:
© 2016, Taylor and Francis Ltd. All rights reserved.

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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