Telomeres are the sequences that form the ends of eukaryotic chromosomes and are essential structures that confer genome stability and guide chromosome behavior. In addition, the terminal regions of the chromosomes tend to house genes with predicted roles in ecological adaptation. Unfortunately, however, most fungal genome assemblies contain very few telomeres and, therefore, the identities of genes residing near the chromosome ends are often unknown. In an effort to develop a complete understanding of the organization and gene content of chromosome ends in a number of fungi, we developed efficient methods for the identification and targeted cloning of telomeres. This chapter describes the basic steps and shows exemplary results from the targeted cloning of Epichloë festucae telomeres.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||21|
|State||Published - 2011|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
I would like to acknowledge the technical support of David Thornbury. The development of these methods was supported by grants MCB 0653930 and EF 0523661 from the National Science Foundation.
© 2011, Springer Science+Business Media, LLC.
- Colony blotting
- Genome sequencing
- Southern blotting
ASJC Scopus subject areas
- Molecular Biology