TY - JOUR
T1 - TCR V β8+ T cells prevent development of toxoplasmic encephalitis in BALB/c mice genetically resistant to the disease
AU - Kang, Hoil
AU - Liesenfeld, Oliver
AU - Remington, Jack S.
AU - Claflin, Jennifer
AU - Wang, Xisheng
AU - Suzuki, Yasuhiro
PY - 2003/4/15
Y1 - 2003/4/15
N2 - BALB/c are genetically resistant to development of toxoplasmic encephalitis (TE) when infected with Toxoplasma gondii, whereas CBA/Ca mice are susceptible. We compared TCR V β chain usage in lymphocytes infiltrated into brains between these animals following infection. TCR V β8+ cells were the most frequent T cell population in brains of infected, resistant BALB/c mice, whereas TCR V β6+ T cells were more prevalent than V β8+ T cells in brains of infected, susceptible CBA/Ca mice. Adoptive transfer of V β8+ immune T cells, obtained from infected BALB/c mice, prevented development of TE and mortality in infected athymic nude mice that lack T cells. In contrast, adoptive transfer of V β6+ immune T cells did not prevent development of TE or mortality in the nude mice. The protective activity of V β8+ immune T cells was greater than that of the total V β8- population. In addition, V β8+ immune T cells produced markedly greater amounts of IFN-γ than did the V β8- population after stimulation with tachyzoite lysate Ags in vitro. Thus, V β8+ T cells appear to play a crucial role in the genetic resistance of BALB/c mice against development of TE.
AB - BALB/c are genetically resistant to development of toxoplasmic encephalitis (TE) when infected with Toxoplasma gondii, whereas CBA/Ca mice are susceptible. We compared TCR V β chain usage in lymphocytes infiltrated into brains between these animals following infection. TCR V β8+ cells were the most frequent T cell population in brains of infected, resistant BALB/c mice, whereas TCR V β6+ T cells were more prevalent than V β8+ T cells in brains of infected, susceptible CBA/Ca mice. Adoptive transfer of V β8+ immune T cells, obtained from infected BALB/c mice, prevented development of TE and mortality in infected athymic nude mice that lack T cells. In contrast, adoptive transfer of V β6+ immune T cells did not prevent development of TE or mortality in the nude mice. The protective activity of V β8+ immune T cells was greater than that of the total V β8- population. In addition, V β8+ immune T cells produced markedly greater amounts of IFN-γ than did the V β8- population after stimulation with tachyzoite lysate Ags in vitro. Thus, V β8+ T cells appear to play a crucial role in the genetic resistance of BALB/c mice against development of TE.
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U2 - 10.4049/jimmunol.170.8.4254
DO - 10.4049/jimmunol.170.8.4254
M3 - Article
C2 - 12682259
AN - SCOPUS:0037446524
SN - 0022-1767
VL - 170
SP - 4254
EP - 4259
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -