Technical Note: A Simple, Rapid Assay for α-Amylase in Bovine Pancreatic Juice

J. A. Walker, D. L. Harmon

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


A procedure is described for the determination of α-amylase activity in bovine pancreatic juice. Bovine pancreatic juice was incubated with potato amylopectin for 6 min, and then alkaline potassium ferricyanide was added to stop the enzymatic reaction. Tubes were placed in a boiling water bath for 10 min and then cooled, and 40 mL of distilled water was added. Optical density was read at 420 nm, and the amount of end product (reducing sugars) was determined by comparison with maltose standards. Ten dilutions of bovine pancreatic secretion were assayed to determine the intraassay precision of this method, resulting in a CV of 1.05%. A pooled sample was assayed five times over five separate days to determine the interassay precision, resulting in a CV of 7.3%. This α-amylase assay provides a repeatable, rapid, and economical determination of α-amylase activity from bovine pancreatic juice.

Original languageEnglish
Pages (from-to)658-662
Number of pages5
JournalJournal of Animal Science
Issue number3
StatePublished - 1996


  • Assays
  • Carbohydrates
  • Digestion
  • Enzymes
  • α-Amylase

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics


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