Tecniche di inseminazione a basso dosaggio per via isteroscopica nella fattrice

Over the past several years, there has been considerable interest in techniques to reduce the number of progressively motile equine spermatozoa needed for successful artificial insemination of the mare. In particular, techniques utilizing sex-sorted spermatozoa and/or cryopreserved spermatozoa would have a greatly enhanced efficiency if the number of spermatozoa required for insemination were reduced. Studies based upon hysteroscopic visualization of the utero-tubal junction (UTJ) and deposition of low sperm numbers (10 × 10⁶ or less) directly onto the ostium of the UTJ demonstrated that the number of spermatozoa required for successful artificial insemination could be reduced by a factor of at least 50 fold. Subsequent studies also demonstrate that deep intrauterine insemination techniques based upon manual techniques may also effectively reduce the number of spermatozoa required for successful artificial insemination of the mare.