Temperature dependence of arginine kinase reaction in the tail muscle of live Sycionia ingentis as measured in vivo by 31P-NMR driven saturation transfer

Teresa W.M. Fan; Richard M. Higashi; Andrew N. Lane

doi:10.1016/0167-4889(92)90164-7

Temperature dependence of arginine kinase reaction in the tail muscle of live Sycionia ingentis as measured in vivo by ³¹P-NMR driven saturation transfer

Teresa W.M. Fan, Richard M. Higashi, Andrew N. Lane

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We have employed the driven ³¹P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.

Original language	English
Pages (from-to)	44-49
Number of pages	6
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1135
Issue number	1
DOIs	https://doi.org/10.1016/0167-4889(92)90164-7
State	Published - Apr 30 1992

Bibliographical note

Funding Information:
The authors are grateful for the financial support (rum the U.S. Departmenotf Interior grant No. 14-35-flll~ll-3f1471W. e wish to also thank Dr. W.H. Clark and his team. ~.~,peciallyD r. Fred Griffin. Athula Wikra-manayakca, nd Philfip Hertzler for their generousg ift of the shrimp. Finally. we are in debt to Dr. Gary Cherr for his valuables uggestions.

Keywords

(Sycionia ingentis)
Arginine kinase
NMR, P-
Tail muscle

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/0167-4889(92)90164-7

Cite this

@article{85f285e82ac34565a90ed042a7ac7f5e,

title = "Temperature dependence of arginine kinase reaction in the tail muscle of live Sycionia ingentis as measured in vivo by 31P-NMR driven saturation transfer",

abstract = "We have employed the driven 31P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.",

keywords = "(Sycionia ingentis), Arginine kinase, NMR, P-, Tail muscle",

author = "Fan, {Teresa W.M.} and Higashi, {Richard M.} and Lane, {Andrew N.}",

note = "Funding Information: The authors are grateful for the financial support (rum the U.S. Departmenotf Interior grant No. 14-35-flll~ll-3f1471W. e wish to also thank Dr. W.H. Clark and his team. ~.~,peciallyD r. Fred Griffin. Athula Wikra-manayakca, nd Philfip Hertzler for their generousg ift of the shrimp. Finally. we are in debt to Dr. Gary Cherr for his valuables uggestions.",

year = "1992",

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doi = "10.1016/0167-4889(92)90164-7",

language = "English",

volume = "1135",

pages = "44--49",

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Temperature dependence of arginine kinase reaction in the tail muscle of live Sycionia ingentis as measured in vivo by ³¹P-NMR driven saturation transfer. / Fan, Teresa W.M.; Higashi, Richard M.; Lane, Andrew N.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1135, No. 1, 30.04.1992, p. 44-49.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Temperature dependence of arginine kinase reaction in the tail muscle of live Sycionia ingentis as measured in vivo by 31P-NMR driven saturation transfer

AU - Fan, Teresa W.M.

AU - Higashi, Richard M.

AU - Lane, Andrew N.

N1 - Funding Information: The authors are grateful for the financial support (rum the U.S. Departmenotf Interior grant No. 14-35-flll~ll-3f1471W. e wish to also thank Dr. W.H. Clark and his team. ~.~,peciallyD r. Fred Griffin. Athula Wikra-manayakca, nd Philfip Hertzler for their generousg ift of the shrimp. Finally. we are in debt to Dr. Gary Cherr for his valuables uggestions.

PY - 1992/4/30

Y1 - 1992/4/30

N2 - We have employed the driven 31P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.

AB - We have employed the driven 31P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.

KW - (Sycionia ingentis)

KW - Arginine kinase

KW - NMR, P-

KW - Tail muscle

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JO - Biochimica et Biophysica Acta - Molecular Cell Research

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