We have employed the driven 31P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.
|Number of pages||6|
|Journal||Biochimica et Biophysica Acta - Molecular Cell Research|
|State||Published - Apr 30 1992|
Bibliographical noteFunding Information:
The authors are grateful for the financial support (rum the U.S. Departmenotf Interior grant No. 14-35-flll~ll-3f1471W. e wish to also thank Dr. W.H. Clark and his team. ~.~,peciallyD r. Fred Griffin. Athula Wikra-manayakca, nd Philfip Hertzler for their generousg ift of the shrimp. Finally. we are in debt to Dr. Gary Cherr for his valuables uggestions.
- (Sycionia ingentis)
- Arginine kinase
- NMR, P-
- Tail muscle
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology