Abstract
We have employed the driven 31P-NMR saturation transfer method to measure in vivo the temperature dependence of the forward and reverse unidirectional fluxes of the arginine kinase reaction in the tail muscle of a live shrimp, Sycionia ingentis. Our results indicated that neither the forward nor the reverse rate constants of this reaction were significantly temperature-dependent between 8 and 16°C, in contrast to the kinetic characteristics of isolated arginine kinases.
Original language | English |
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Pages (from-to) | 44-49 |
Number of pages | 6 |
Journal | Biochimica et Biophysica Acta - Molecular Cell Research |
Volume | 1135 |
Issue number | 1 |
DOIs | |
State | Published - Apr 30 1992 |
Bibliographical note
Funding Information:The authors are grateful for the financial support (rum the U.S. Departmenotf Interior grant No. 14-35-flll~ll-3f1471W. e wish to also thank Dr. W.H. Clark and his team. ~.~,peciallyD r. Fred Griffin. Athula Wikra-manayakca, nd Philfip Hertzler for their generousg ift of the shrimp. Finally. we are in debt to Dr. Gary Cherr for his valuables uggestions.
Keywords
- (Sycionia ingentis)
- Arginine kinase
- NMR, P-
- Tail muscle
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology