TY - JOUR
T1 - Temporomandibular joint nociception
T2 - Effects of capsaicin on substance P-like immunoreactivity in the rabbit brain stem
AU - Kyrkanides, Stephanos
AU - Tallents, Ross H.
AU - Macher, Donald J.
AU - Olschowka, John A.
AU - Stevens, Suzanne Y.
PY - 2002
Y1 - 2002
N2 - Aims: To specify the regions of the brain stem that are characterized by changes in substance P (SP)-like immunoreactivity following activation of capsaicin-sensitive afferents innervating temporomandibular joint (TMJ) tissues in New Zealand rabbits. Methods: Capsaicin, an activator of small-diameter unmyelinated and thinly myelinated nociceptive afferent fibers, was administered unilaterally to the right TMJ of experimental animals. Another group received vehicle solution and served as controls. The animals were sacrificed 6 hours post-treatment through transcardial perfusion. Their brain stems were removed and sectioned, and SP-like immunoreactivity was assessed in serial horizontal sections. Results: A decrease in brain stem SP-like immunoreactivity occurred ipsilateral to capsaicin application. This reduction was primarily localized in brain stem regions that correspond to the trigeminal main sensory nucleus, as well as subnucleus oralis, interpolaris, and caudalis of the trigeminal spinal tract nucleus. Conclusion: The present study revealed central nervous system changes following TMJ capsaicin treatment in rabbits.
AB - Aims: To specify the regions of the brain stem that are characterized by changes in substance P (SP)-like immunoreactivity following activation of capsaicin-sensitive afferents innervating temporomandibular joint (TMJ) tissues in New Zealand rabbits. Methods: Capsaicin, an activator of small-diameter unmyelinated and thinly myelinated nociceptive afferent fibers, was administered unilaterally to the right TMJ of experimental animals. Another group received vehicle solution and served as controls. The animals were sacrificed 6 hours post-treatment through transcardial perfusion. Their brain stems were removed and sectioned, and SP-like immunoreactivity was assessed in serial horizontal sections. Results: A decrease in brain stem SP-like immunoreactivity occurred ipsilateral to capsaicin application. This reduction was primarily localized in brain stem regions that correspond to the trigeminal main sensory nucleus, as well as subnucleus oralis, interpolaris, and caudalis of the trigeminal spinal tract nucleus. Conclusion: The present study revealed central nervous system changes following TMJ capsaicin treatment in rabbits.
KW - Immunocytochemistry
KW - Nociception
KW - Rabbit
KW - Substance P
KW - Temporomandibular joint
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M3 - Article
C2 - 12221739
AN - SCOPUS:0036362409
SN - 1064-6655
VL - 16
SP - 229
EP - 236
JO - Journal of Orofacial Pain
JF - Journal of Orofacial Pain
IS - 3
ER -