Tetracycline desorption kinetics in municipal biosolids and poultry litter amendments determined by diffusive gradients in thin films (DGT)

E. D'Angelo, Angela Martin

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Tetracycline (TET) is commonly used to treat bacterial diseases in humans and chickens (Gallus gallus domesticus), is largely excreted, and is often found at elevated concentrations in treated sewage sludge (biosolids) and poultry litter (excrement plus bedding materials). Land spreading of these materials is practiced worldwide to improve soil fertility, but the practice raises questions about whether TET could be released to the environment and cause adverse effects. Hazard risks largely depend on the concentration in the solid phase that can be released to the solution phase (labile TET), it's desorption rate constant, and diffusion rate of dissolved TET in amendments. In this study, these quantities were evaluated in biosolids and three types of litter amendments by combinations of equilibrium sorption-desorption isotherm and desorption kinetic studies using diffusive gradient in thin films (DGT) samplers. Results from isotherm experiments showed that TET partitioning was inhibited at the high dissolved organic carbon (DOC) concentrations in amendments (6–15% of dry mass). Despite low partition coefficients determined at high particle/DOC concentrations of amendments (Kd = 9–46 mL g−1), results from DGT experiments revealed that TET release by desorption and diffusion would be slow and short-lived (<3 d) due to small effective diffusion coefficients (<8 × 10−8 cm2 s−1) and low concentrations of labile TET in amendments (<5% of total TET). Despite this, evaluations of antibiotic uptake during microbial colonization and plant root interception of amendment surfaces are highly warranted.

Original languageEnglish
Pages (from-to)232-239
Number of pages8
StatePublished - Oct 2018

Bibliographical note

Funding Information:
Acknowledgements : The authors express sincere appreciation for research support from the Hatch Multistate Research Fund (MRF) provided by National Institute of Food and Agriculture for project W3170-Beneficial Reuse of Residuals and Reclaimed Water: Impact on Soil Ecosystem and Human Health. The authors would also like to express their sincere gratitude to Drs. T. Pescatore, M. Ford, and Edwin Ritchey for providing poultry manure and litter used in this project, and Dr. Niklas Lehto for sharing the software tool 2D-DIFS that was essential for determining the kinetic parameters in this study.

Publisher Copyright:
© 2018 Elsevier Ltd


  • 2D-DIFS
  • Dissolved organic carbon
  • Effective diffusion coefficient
  • First-order desorption rate constant
  • Labile concentration
  • Sorption-desorption partition constant

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry (all)
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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