TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

Ahmed Abdel-Latif; Ewa K. Zuba-Surma; Jamie Case; Sumit Tiwari; Greg Hunt; Smita Ranjan; Robert J. Vincent; Edward F. Srour; Roberto Bolli; Buddhadeb Dawn

doi:10.1007/s00395-008-0729-9

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

Ahmed Abdel-Latif
, Ewa K. Zuba-Surma
, Jamie Case
, Sumit Tiwari
, Greg Hunt
, Smita Ranjan
, Robert J. Vincent
, Edward F. Srour
, Roberto Bolli
, Buddhadeb Dawn

Internal Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

Original language	English
Pages (from-to)	514-524
Number of pages	11
Journal	Basic Research in Cardiology
Volume	103
Issue number	6
DOIs	https://doi.org/10.1007/s00395-008-0729-9
State	Published - 2008

Bibliographical note

Funding Information:
We gratefully acknowledge Barbara Turgeon for expert secretarial assistance. This study was supported in part by NIH grants R01 HL-72410, HL-55757, HL-68088, HL-70897, HL-76794, HL-78825, and R21 HL-89737.

Funding

We gratefully acknowledge Barbara Turgeon for expert secretarial assistance. This study was supported in part by NIH grants R01 HL-72410, HL-55757, HL-68088, HL-70897, HL-76794, HL-78825, and R21 HL-89737.

Funders	Funder number
National Institutes of Health (NIH)	HL-70897, R21 HL-89737, R01 HL-72410, HL-78825, HL-55757, HL-68088
National Heart, Lung, and Blood Institute (NHLBI)	R01HL076794

Keywords

Adult stem cell
Cardiac differentiation
Culture medium
Growth factor
Skeletal muscle

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1007/s00395-008-0729-9

Cite this

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title = "TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells",

abstract = "The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.",

keywords = "Adult stem cell, Cardiac differentiation, Culture medium, Growth factor, Skeletal muscle",

author = "Ahmed Abdel-Latif and Zuba-Surma, \{Ewa K.\} and Jamie Case and Sumit Tiwari and Greg Hunt and Smita Ranjan and Vincent, \{Robert J.\} and Srour, \{Edward F.\} and Roberto Bolli and Buddhadeb Dawn",

note = "Funding Information: We gratefully acknowledge Barbara Turgeon for expert secretarial assistance. This study was supported in part by NIH grants R01 HL-72410, HL-55757, HL-68088, HL-70897, HL-76794, HL-78825, and R21 HL-89737.",

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T1 - TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

AU - Abdel-Latif, Ahmed

AU - Zuba-Surma, Ewa K.

AU - Case, Jamie

AU - Tiwari, Sumit

AU - Hunt, Greg

AU - Ranjan, Smita

AU - Vincent, Robert J.

AU - Srour, Edward F.

AU - Bolli, Roberto

AU - Dawn, Buddhadeb

N1 - Funding Information: We gratefully acknowledge Barbara Turgeon for expert secretarial assistance. This study was supported in part by NIH grants R01 HL-72410, HL-55757, HL-68088, HL-70897, HL-76794, HL-78825, and R21 HL-89737.

PY - 2008

Y1 - 2008

N2 - The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

AB - The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

KW - Adult stem cell

KW - Cardiac differentiation

KW - Culture medium

KW - Growth factor

KW - Skeletal muscle

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JF - Basic Research in Cardiology

IS - 6

ER -

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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