The 5′ untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells

Zhaiyi Zhang; Marina Falaleeva; Lily Agranat-Tamir; Amadis Pages; Eduardo Eyras; Joseph Sperling; Ruth Sperling; Stefan Stamm

doi:10.1007/s00221-013-3458-8

The 5′ untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells

Zhaiyi Zhang, Marina Falaleeva, Lily Agranat-Tamir, Amadis Pages, Eduardo Eyras, Joseph Sperling, Ruth Sperling, Stefan Stamm

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled receptor that is exclusively expressed in neurons. Here, we report that the 5′ untranslated region of the receptor pre-mRNA as well as its hosted miRNAs is widely expressed in non-neuronal cell lines. Alternative splicing of HTR2C is regulated by MBII-52. MBII-52 and the neighboring MBII-85 cluster are absent in people with Prader-Willi syndrome, which likely causes the disease. We show that MBII-52 and MBII-85 increase expression of the HTR2C 5′ UTR and influence expression of the hosted miRNAs. The data indicate that the transcriptional unit expressing HTR2C is more complex than previously recognized and likely deregulated in Prader-Willi syndrome.

Original language	English
Pages (from-to)	387-394
Number of pages	8
Journal	Experimental Brain Research
Volume	230
Issue number	4
DOIs	https://doi.org/10.1007/s00221-013-3458-8
State	Published - Oct 2013

Bibliographical note

Funding Information:
Acknowledgments this work was supported by nIH RO1 GM083187, P20RR020171 to SS; GM079549 to RS and JS; Binational Science Foundation (BSF), USA-Israel, transformative Grant, # 2010508, to SS and RS. EE and AP were supported by the Spanish Ministry of Science with grant BIO2011-23920 and by Sandra Ibarra Foundation for Cancer with grant FSI 2011-035.

Keywords

Alternative splicing
Serotonin receptor
miRNA
snoRNA

ASJC Scopus subject areas

General Neuroscience

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10.1007/s00221-013-3458-8

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title = "The 5′ untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells",

abstract = "The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled receptor that is exclusively expressed in neurons. Here, we report that the 5′ untranslated region of the receptor pre-mRNA as well as its hosted miRNAs is widely expressed in non-neuronal cell lines. Alternative splicing of HTR2C is regulated by MBII-52. MBII-52 and the neighboring MBII-85 cluster are absent in people with Prader-Willi syndrome, which likely causes the disease. We show that MBII-52 and MBII-85 increase expression of the HTR2C 5′ UTR and influence expression of the hosted miRNAs. The data indicate that the transcriptional unit expressing HTR2C is more complex than previously recognized and likely deregulated in Prader-Willi syndrome.",

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author = "Zhaiyi Zhang and Marina Falaleeva and Lily Agranat-Tamir and Amadis Pages and Eduardo Eyras and Joseph Sperling and Ruth Sperling and Stefan Stamm",

note = "Funding Information: Acknowledgments this work was supported by nIH RO1 GM083187, P20RR020171 to SS; GM079549 to RS and JS; Binational Science Foundation (BSF), USA-Israel, transformative Grant, # 2010508, to SS and RS. EE and AP were supported by the Spanish Ministry of Science with grant BIO2011-23920 and by Sandra Ibarra Foundation for Cancer with grant FSI 2011-035.",

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doi = "10.1007/s00221-013-3458-8",

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AU - Zhang, Zhaiyi

AU - Falaleeva, Marina

AU - Agranat-Tamir, Lily

AU - Pages, Amadis

AU - Eyras, Eduardo

AU - Sperling, Joseph

AU - Sperling, Ruth

AU - Stamm, Stefan

N1 - Funding Information: Acknowledgments this work was supported by nIH RO1 GM083187, P20RR020171 to SS; GM079549 to RS and JS; Binational Science Foundation (BSF), USA-Israel, transformative Grant, # 2010508, to SS and RS. EE and AP were supported by the Spanish Ministry of Science with grant BIO2011-23920 and by Sandra Ibarra Foundation for Cancer with grant FSI 2011-035.

PY - 2013/10

Y1 - 2013/10

N2 - The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled receptor that is exclusively expressed in neurons. Here, we report that the 5′ untranslated region of the receptor pre-mRNA as well as its hosted miRNAs is widely expressed in non-neuronal cell lines. Alternative splicing of HTR2C is regulated by MBII-52. MBII-52 and the neighboring MBII-85 cluster are absent in people with Prader-Willi syndrome, which likely causes the disease. We show that MBII-52 and MBII-85 increase expression of the HTR2C 5′ UTR and influence expression of the hosted miRNAs. The data indicate that the transcriptional unit expressing HTR2C is more complex than previously recognized and likely deregulated in Prader-Willi syndrome.

AB - The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled receptor that is exclusively expressed in neurons. Here, we report that the 5′ untranslated region of the receptor pre-mRNA as well as its hosted miRNAs is widely expressed in non-neuronal cell lines. Alternative splicing of HTR2C is regulated by MBII-52. MBII-52 and the neighboring MBII-85 cluster are absent in people with Prader-Willi syndrome, which likely causes the disease. We show that MBII-52 and MBII-85 increase expression of the HTR2C 5′ UTR and influence expression of the hosted miRNAs. The data indicate that the transcriptional unit expressing HTR2C is more complex than previously recognized and likely deregulated in Prader-Willi syndrome.

KW - Alternative splicing

KW - Serotonin receptor

KW - miRNA

KW - snoRNA

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The 5′ untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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