The Arabidopsis mutant eer2 has enhanced ethylene responses in the light

Annelies De Paepe; Liesbeth De Grauwe; Sophie Bertrand; Jan Smalle; Dominique Van Der Straeten

doi:10.1093/jxb/eri234

The Arabidopsis mutant eer2 has enhanced ethylene responses in the light

Annelies De Paepe, Liesbeth De Grauwe, Sophie Bertrand, Jan Smalle, Dominique Van Der Straeten

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

By screening for ethylene response mutants in Arabidopsis, a novel mutant, eer2, was isolated which 0displays enhanced ethylene responses. On a low nutrient medium (LNM) light-grown eer2 seedlings showed a significant hypocotyl elongation in response to low levels of 1-amino-cyclopropane-1-carboxylate (ACC), the precursor of ethylene, compared with the wild type, indicating that eer2 is hypersensitive to ethylene. Treatment with 1-MCP (1-methylcyclopropene), a competitive inhibitor of ethylene signalling, suppressed this hypersensitive response, demonstrating that it is a bona fide ethylene effect. By contrast, roots of eer2 were less sensitive than the wild type to low concentrations of ACC. The ethylene levels in eer2 did not differ from the wild type, indicating that ethylene over-production is not the primary cause of the eer2 phenotype. In addition to its enhanced ethylene response of hypocotyls, eer2 is also affected in the pattern of senescence and its phenotype depends on the nutritional status of the growth medium. Furthermore, linkage analysis of eer2 suggests that this mutant defines a new locus in ethylene signalling.

Original language	English
Pages (from-to)	2409-2420
Number of pages	12
Journal	Journal of Experimental Botany
Volume	56
Issue number	419
DOIs	https://doi.org/10.1093/jxb/eri234
State	Published - Sep 2005

Bibliographical note

Funding Information:
We thank Peter Lootens and Paul Vandecasteele from the Agricultural Research Centre in Merelbeke for the training and use of the portable fluorometer PAM-2000, Mira Haegman and Piet Vankeirsbilck for technical assistance with the ethylene measurements, Jan Goeman and Johan Vandereycken for supplying 1-MCP, Andrea Miyasaka de Almeida for critical reading, and Tom Gerats, Janny Peeters, and Marnik Vuylsteke for helpful discussions. This work was supported by a PhD fellowship to Annelies De Paepe and research grants to Dominique Van Der Straeten (G.0281.98, WO.004.99, and G.0345.02) from the Fund for Scientific Research (Flanders).

Keywords

Arabidopsis
Enhanced response
Ethylene
Hypocotyl
Metal transport
Metal uptake
Senescence

ASJC Scopus subject areas

Physiology
Plant Science

Access to Document

10.1093/jxb/eri234

Cite this

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title = "The Arabidopsis mutant eer2 has enhanced ethylene responses in the light",

abstract = "By screening for ethylene response mutants in Arabidopsis, a novel mutant, eer2, was isolated which 0displays enhanced ethylene responses. On a low nutrient medium (LNM) light-grown eer2 seedlings showed a significant hypocotyl elongation in response to low levels of 1-amino-cyclopropane-1-carboxylate (ACC), the precursor of ethylene, compared with the wild type, indicating that eer2 is hypersensitive to ethylene. Treatment with 1-MCP (1-methylcyclopropene), a competitive inhibitor of ethylene signalling, suppressed this hypersensitive response, demonstrating that it is a bona fide ethylene effect. By contrast, roots of eer2 were less sensitive than the wild type to low concentrations of ACC. The ethylene levels in eer2 did not differ from the wild type, indicating that ethylene over-production is not the primary cause of the eer2 phenotype. In addition to its enhanced ethylene response of hypocotyls, eer2 is also affected in the pattern of senescence and its phenotype depends on the nutritional status of the growth medium. Furthermore, linkage analysis of eer2 suggests that this mutant defines a new locus in ethylene signalling.",

keywords = "Arabidopsis, Enhanced response, Ethylene, Hypocotyl, Metal transport, Metal uptake, Senescence",

author = "{De Paepe}, Annelies and {De Grauwe}, Liesbeth and Sophie Bertrand and Jan Smalle and {Van Der Straeten}, Dominique",

note = "Funding Information: We thank Peter Lootens and Paul Vandecasteele from the Agricultural Research Centre in Merelbeke for the training and use of the portable fluorometer PAM-2000, Mira Haegman and Piet Vankeirsbilck for technical assistance with the ethylene measurements, Jan Goeman and Johan Vandereycken for supplying 1-MCP, Andrea Miyasaka de Almeida for critical reading, and Tom Gerats, Janny Peeters, and Marnik Vuylsteke for helpful discussions. This work was supported by a PhD fellowship to Annelies De Paepe and research grants to Dominique Van Der Straeten (G.0281.98, WO.004.99, and G.0345.02) from the Fund for Scientific Research (Flanders).",

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AU - De Paepe, Annelies

AU - De Grauwe, Liesbeth

AU - Bertrand, Sophie

AU - Smalle, Jan

AU - Van Der Straeten, Dominique

N1 - Funding Information: We thank Peter Lootens and Paul Vandecasteele from the Agricultural Research Centre in Merelbeke for the training and use of the portable fluorometer PAM-2000, Mira Haegman and Piet Vankeirsbilck for technical assistance with the ethylene measurements, Jan Goeman and Johan Vandereycken for supplying 1-MCP, Andrea Miyasaka de Almeida for critical reading, and Tom Gerats, Janny Peeters, and Marnik Vuylsteke for helpful discussions. This work was supported by a PhD fellowship to Annelies De Paepe and research grants to Dominique Van Der Straeten (G.0281.98, WO.004.99, and G.0345.02) from the Fund for Scientific Research (Flanders).

PY - 2005/9

Y1 - 2005/9

N2 - By screening for ethylene response mutants in Arabidopsis, a novel mutant, eer2, was isolated which 0displays enhanced ethylene responses. On a low nutrient medium (LNM) light-grown eer2 seedlings showed a significant hypocotyl elongation in response to low levels of 1-amino-cyclopropane-1-carboxylate (ACC), the precursor of ethylene, compared with the wild type, indicating that eer2 is hypersensitive to ethylene. Treatment with 1-MCP (1-methylcyclopropene), a competitive inhibitor of ethylene signalling, suppressed this hypersensitive response, demonstrating that it is a bona fide ethylene effect. By contrast, roots of eer2 were less sensitive than the wild type to low concentrations of ACC. The ethylene levels in eer2 did not differ from the wild type, indicating that ethylene over-production is not the primary cause of the eer2 phenotype. In addition to its enhanced ethylene response of hypocotyls, eer2 is also affected in the pattern of senescence and its phenotype depends on the nutritional status of the growth medium. Furthermore, linkage analysis of eer2 suggests that this mutant defines a new locus in ethylene signalling.

AB - By screening for ethylene response mutants in Arabidopsis, a novel mutant, eer2, was isolated which 0displays enhanced ethylene responses. On a low nutrient medium (LNM) light-grown eer2 seedlings showed a significant hypocotyl elongation in response to low levels of 1-amino-cyclopropane-1-carboxylate (ACC), the precursor of ethylene, compared with the wild type, indicating that eer2 is hypersensitive to ethylene. Treatment with 1-MCP (1-methylcyclopropene), a competitive inhibitor of ethylene signalling, suppressed this hypersensitive response, demonstrating that it is a bona fide ethylene effect. By contrast, roots of eer2 were less sensitive than the wild type to low concentrations of ACC. The ethylene levels in eer2 did not differ from the wild type, indicating that ethylene over-production is not the primary cause of the eer2 phenotype. In addition to its enhanced ethylene response of hypocotyls, eer2 is also affected in the pattern of senescence and its phenotype depends on the nutritional status of the growth medium. Furthermore, linkage analysis of eer2 suggests that this mutant defines a new locus in ethylene signalling.

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KW - Metal transport

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ER -

The Arabidopsis mutant eer2 has enhanced ethylene responses in the light

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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