The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Stephen A. Bell; Arthur G. Hunt

doi:10.1016/j.febslet.2010.03.007

The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Stephen A. Bell
, Arthur G. Hunt

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.

Original language	English
Pages (from-to)	1449-1454
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2010.03.007
State	Published - Apr 2010

Bibliographical note

Funding Information:
The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

Funding

The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

Funders	Funder number
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	0817818

Keywords

CPSF30
CstF77
Polyadenylation
RNA-binding

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2010.03.007

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title = "The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein",

abstract = "The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.",

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author = "Bell, \{Stephen A.\} and Hunt, \{Arthur G.\}",

note = "Funding Information: The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.",

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AU - Bell, Stephen A.

AU - Hunt, Arthur G.

N1 - Funding Information: The authors are grateful to Carol Von Laken for logistical support and to Drs. Quinn Li, Suryadevara Rao, and Balasubrahmanyam Addepalli for clones and reagents. This research was supported by NSF Grant IOS-0817818.

PY - 2010/4

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N2 - The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.

AB - The 77. kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3′ end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30. kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting this interaction, it was found that the C-terminus of AtCstF77 interacts with AtCPSF30. Remarkably, we also found that the C-terminal domain of AtCstF77 possesses RNA-binding ability. These studies therefore reveal AtCstF77 to be an RNA-binding protein, adding yet another RNA-binding activity to the plant polyadenylation complex. This raises interesting questions as to the means by which RNAs are recognized during mRNA 3′ end formation in plants.

KW - CPSF30

KW - CstF77

KW - Polyadenylation

KW - RNA-binding

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

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The Arabidopsis ortholog of the 77kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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