The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis

Pieter C. Dorrestein, Steven G. Van Lanen, Wenli Li, Chunhua Zhao, Zixin Deng, Ben Shen, Neil L. Kellener

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The HAD superfamily protein OzmB from the oxazolomycin biosynthetic pathway is shown to divert the primary metabolite 1,3-diphosphoglycerate into the polyketide biosynthetic pathway as glycerate via loading of a carrier protein. Each of the stepsactivation of d-3-phosphoglycerate, dephosphorylation while attached to a cysteine on OzmB, and subsequent transfer of glycerate to the phosphopantetheinyl thiol of an acyl carrier proteinwas monitored by nanospray Fourier transform mass spectrometry. This activation of phosphoglycerate represents a general mechanism of diverting glycolytic metabolites into glyceryl-derived polyketides.

Original languageEnglish
Pages (from-to)10386-10387
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number32
DOIs
StatePublished - Aug 16 2006

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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