The biosynthesis of indolocarbazoles in a heterologous E. coli host

Chang Gu Hyun, Tsion Bililign, Jianchun Liao, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


The antitumor antibiotic rebeccamycin (1), produced by the bacterium Saccharothrix aerocolonigenes, is a prototype of a class of complex natural products called indolocarbazoles. The cloning and characterization of the rebeccamcyin (reb) gene locus from S. aerocolonigenes is reported. The heterologous expression of this intact gene cluster in Escherichia coli led to the production of 1 and two putative biosynthetic intermediates. This work represents the first production of indolocarbazole analogues in the heterologous host E. coli.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
Issue number1
StatePublished - Jan 3 2003


  • Biosynthesis
  • Gene locus
  • Heterologous expression
  • Natural products
  • Rebeccamycin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry


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