The biosynthesis of liposidomycin-like A-90289 antibiotics featuring a new type of sulfotransferase

Masanori Funabashi, Satoshi Baba, Koichi Nonaka, Masahiko Hosobuchi, Yoko Fujita, Tomoyuki Shibata, Steven G. Van Lanen

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Lip reading: The biosynthetic gene cluster for A-90289, a fatty acid nucleoside antibiotic, was cloned and sequenced. The sulfotransferase LipB is demonstrated to be essential for A-90289 biosynthesis, and in vitro characterization revealed LipB utilizes p-nitrophenylsulfate as an aryl sulfate donor and a variety of nonaryl acceptors including caprazamycin A to give the 2′-O-sulfated product, A-90289A. (Chemical Equation Presented).

Original languageEnglish
Pages (from-to)184-190
Number of pages7
JournalChemBioChem
Volume11
Issue number2
DOIs
StatePublished - Jan 25 2010

Keywords

  • Antibiotics
  • Bacterial translocase I
  • Biosynthesis
  • Nucleosides
  • Sulfotransferase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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