The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710

Qunjie Gao, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Dynemicin is a novel anthraquinone-fused member of the 10-membered enediyne antitumor antibiotic family. The development of a genetic system for the dynemicin producer Micromonospora chersina confirmed, for the first time, the requirement of the putative enediyne core biosynthetic genes (dynE8, U14 and U15) and a tailoring oxidase gene (orf23) for dynemicin production. Cloning and sequence analysis of a 76 kb of genomic sequence region containing dynE8 revealed a variety of genes conserved among known enediyne loci. Surprisingly, this fragment and flanking chromosomal DNA lacked any obvious genes encoding for the biosynthesis of the anthraquinone, suggesting that the location of genes encoding for the biosynthesis of the dynemicin enediyne core and the dynemicin anthraquinone are chromosomally distinct. The demonstrated trace production of a shunt product from mutant strain QGD23 (Δorf23) also sets the stage for subsequent studies to delineate the key steps in enediyne core biosynthesis and tailoring.

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalFEMS Microbiology Letters
Volume282
Issue number1
DOIs
StatePublished - May 2008

Keywords

  • Biosynthesis
  • Cancer
  • Dynemicin
  • Enediyne
  • Gene disruption
  • Polyketide synthase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710'. Together they form a unique fingerprint.

Cite this