The calicheamicin gene cluster and its iterative type I enediyne PKS

Joachim Ahlert; Erica Shepard; Natalia Lomovskaya; Emmanuel Zazopoulos; Alfredo Staffa; Brian O. Bachmann; Kexue Huang; Leonid Fonstein; Anne Czisny; Ross E. Whitwam; Chris M. Farnet; Jon S. Thorson

doi:10.1126/science.1072105

The calicheamicin gene cluster and its iterative type I enediyne PKS

Joachim Ahlert, Erica Shepard, Natalia Lomovskaya, Emmanuel Zazopoulos, Alfredo Staffa, Brian O. Bachmann, Kexue Huang, Leonid Fonstein, Anne Czisny, Ross E. Whitwam, Chris M. Farnet, Jon S. Thorson

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Abstract

The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

Original language	English
Pages (from-to)	1173-1176
Number of pages	4
Journal	Science
Volume	297
Issue number	5584
DOIs	https://doi.org/10.1126/science.1072105
State	Published - Aug 16 2002

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title = "The calicheamicin gene cluster and its iterative type I enediyne PKS",

abstract = "The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.",

author = "Joachim Ahlert and Erica Shepard and Natalia Lomovskaya and Emmanuel Zazopoulos and Alfredo Staffa and Bachmann, {Brian O.} and Kexue Huang and Leonid Fonstein and Anne Czisny and Whitwam, {Ross E.} and Farnet, {Chris M.} and Thorson, {Jon S.}",

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T1 - The calicheamicin gene cluster and its iterative type I enediyne PKS

AU - Ahlert, Joachim

AU - Shepard, Erica

AU - Lomovskaya, Natalia

AU - Zazopoulos, Emmanuel

AU - Staffa, Alfredo

AU - Bachmann, Brian O.

AU - Huang, Kexue

AU - Fonstein, Leonid

AU - Czisny, Anne

AU - Whitwam, Ross E.

AU - Farnet, Chris M.

AU - Thorson, Jon S.

PY - 2002/8/16

Y1 - 2002/8/16

N2 - The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

AB - The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

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The calicheamicin gene cluster and its iterative type I enediyne PKS

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