The calicheamicin gene cluster and its iterative type I enediyne PKS

Joachim Ahlert, Erica Shepard, Natalia Lomovskaya, Emmanuel Zazopoulos, Alfredo Staffa, Brian O. Bachmann, Kexue Huang, Leonid Fonstein, Anne Czisny, Ross E. Whitwam, Chris M. Farnet, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

239 Scopus citations

Abstract

The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

Original languageEnglish
Pages (from-to)1173-1176
Number of pages4
JournalScience
Volume297
Issue number5584
DOIs
StatePublished - Aug 16 2002

ASJC Scopus subject areas

  • General

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