The carboxy terminus causes interfacial assembly of oleate hydratase on a membrane bilayer

Funding

We thank Amanda Nourse in the St Jude Children’s Research Hospital (SJCRH) Molecular Interaction Analysis Shared Resource for sample analysis by SEC-MALS. We thank Sagar Chittori and Syed Asfarul Haque in the SJCRH Cryo-Electron Microscopy Center for assistance with cryo-EM data collection. Super resolution microscopy images were acquired at the SJCRH Cell & Tissue Imaging Center, which is supported by NCI P30 CA021765 . The OhyA CTD peptide(550–591) was synthesized in the Macromolecular Synthesis Laboratory (Hartwell Center of Biotechnology, St Jude Children’s Research Hospital). This work was supported by National Institutes of Health Grants R01-GM034496 (C. O. R.), R00-AI166116 (C. D. R.), R01-GM123455 , P41-GM104601 , and R24-GM145965 (E. T.), Cancer Center Support Grant CA21765 , and the American Lebanese Syrian Associated Charities . The simulations reported in this study are supported by XSEDE allocation (grant MCA06N060 to E. T.), Microsoft Azure , and Blue Waters at the National Center for Supercomputing Application (NCSA) at the University of Illinois at Urbana-Champaign . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We thank Amanda Nourse in the St Jude Children's Research Hospital (SJCRH) Molecular Interaction Analysis Shared Resource for sample analysis by SEC-MALS. We thank Sagar Chittori and Syed Asfarul Haque in the SJCRH Cryo-Electron Microscopy Center for assistance with cryo-EM data collection. Super resolution microscopy images were acquired at the SJCRH Cell & Tissue Imaging Center, which is supported by NCI P30 CA021765. The OhyA CTD peptide(550–591) was synthesized in the Macromolecular Synthesis Laboratory (Hartwell Center of Biotechnology, St Jude Children's Research Hospital). C. D. R. and C. O. R. conceptualization; C. D. R. C. R. G. H. S. H. Y. L. C. C. R. M. L. O. M. Z. Q. A. P. R. C. K. E. T. and C. O. R. formal analysis; C. D. R. C. R. G. H. S. H. Y. L. C. C. R. M. L. O. M. Z. Q. A. P. R. C. K. E. T. and C. O. R. investigation, C. D. R. C. R. G. H. S. H, Y. L. C. C. R. E. T. P. R. M. L. O. M. Z. Q. R. C. K. A. P. and W. J. M. resources; C. D. R. writing–original draft; C. D. R. C. R. G. H. S. H. Y. L. C. C. R. P. R. M. L. O. M. Z. Q, A. P. W. J. M. R. C. K. E. T. and C. O. R. writing–reviewing and editing; R. C. K. E. T. and C. O. R. resources; C. D. R. visualization and project administration; C. D. R. E. T. and C. O. R. funding acquisition. This work was supported by National Institutes of Health Grants R01-GM034496 (C. O. R.), R00-AI166116 (C. D. R.), R01-GM123455, P41-GM104601, and R24-GM145965 (E. T.), Cancer Center Support Grant CA21765, and the American Lebanese Syrian Associated Charities. The simulations reported in this study are supported by XSEDE allocation (grant MCA06N060 to E. T.), Microsoft Azure, and Blue Waters at the National Center for Supercomputing Application (NCSA) at the University of Illinois at Urbana-Champaign. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.