The cardiomyocyte molecular clock regulates the circadian expression of Kcnh2 and contributes to ventricular repolarization

Elizabeth A. Schroder, Don E. Burgess, Xiping Zhang, Mellani Lefta, Jennifer L. Smith, Abhijit Patwardhan, Daniel C. Bartos, Claude S. Elayi, Karyn A. Esser, Brian P. Delisle

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Background Sudden cardiac death (SCD) follows a diurnal variation. Data suggest the timing of SCD is influenced by circadian (~24-hour) changes in neurohumoral and cardiomyocyte-specific regulation of the heart's electrical properties. The basic helix-loop-helix transcription factors brain muscle arnt-like1 (BMAL1) and circadian locomotor output control kaput (CLOCK) coordinate the circadian expression of select genes. Objective We sought to test whether Bmal1 expression in cardiomyocytes contributes to K+ channel expression and diurnal changes in ventricular repolarization. Methods We used transgenic mice that allow for the inducible cardiomyocyte-specific deletion of Bmal1 (iCSΔBmal1-/-). We used quantitative polymerase chain reaction, voltage clamping, promoter-reporter bioluminescence assays, and electrocardiographic telemetry. Results Although several K+ channel gene transcripts were downregulated in iCSΔBmal1-/-mouse hearts, only Kcnh2 exhibited a robust circadian pattern of expression that was disrupted in iCSΔBmal1-/- hearts. Kcnh2 underlies the rapidly activating delayed-rectifier K+ current, and the rapidly activating delayed-rectifier K+ current recorded from iCSΔBmal1-/- ventricular cardiomyocytes was ~50% smaller than control ventricular myocytes. Promoter-reporter assays demonstrated that the human Kcnh2 promoter is transactivated by the coexpression of BMAL1 and CLOCK. Electrocardiographic analysis showed that iCSΔBmal1-/- mice developed a prolongation in the heart rate-corrected QT interval during the light (resting) phase. This was secondary to an augmented circadian rhythm in the uncorrected QT interval without a corresponding change in the RR interval. Conclusion The molecular clock in the heart regulates the circadian expression of Kcnh2, modifies K+ channel gene expression, and is important for normal ventricular repolarization. Disruption of the cardiomyocyte circadian clock mechanism likely unmasks diurnal changes in ventricular repolarization that could contribute to an increased risk of cardiac arrhythmias/SCD.

Original languageEnglish
Pages (from-to)1306-1314
Number of pages9
JournalHeart Rhythm
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Heart Rhythm Society.

Keywords

  • Bmal1
  • Circadian rhythm
  • ECG
  • Kcnh2
  • QTc
  • Repolarization

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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