TY - JOUR
T1 - The central cell nuclear position at the micropylar end is maintained by the balance of F-actin dynamics, but dispensable for karyogamy in Arabidopsis
AU - Kawashima, Tomokazu
AU - Berger, Frederic
N1 - Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.
PY - 2015/6/16
Y1 - 2015/6/16
N2 - Key message: Advances in Fertilization. In flowering plants, fertilization is initiated by the delivery of immotile sperm cells to the boundary between two female gametes, the egg cell and the central cell. During female gamete development in Arabidopsis, the nucleus of the central cell becomes positioned toward this boundary. How this specific polarized nuclear position is maintained is unclear and whether it plays a role in successful fertilization remains to be determined. By disrupting and manipulating F-actin dynamics in the central cell, we identified that the balance of F-actin dynamics is important for nuclear positioning in the central cell and that the presence of intact F-actin cables in the central cell correlates with successful karyogamy regardless of the central cell nuclear position. We also report that the surface of the central cell nucleus is enriched in F-actin. Thus, the central cell nucleus might also serve as a site that organizes F-actin cytoskeleton to promote sperm cell nucleus movement and karyogamy.
AB - Key message: Advances in Fertilization. In flowering plants, fertilization is initiated by the delivery of immotile sperm cells to the boundary between two female gametes, the egg cell and the central cell. During female gamete development in Arabidopsis, the nucleus of the central cell becomes positioned toward this boundary. How this specific polarized nuclear position is maintained is unclear and whether it plays a role in successful fertilization remains to be determined. By disrupting and manipulating F-actin dynamics in the central cell, we identified that the balance of F-actin dynamics is important for nuclear positioning in the central cell and that the presence of intact F-actin cables in the central cell correlates with successful karyogamy regardless of the central cell nuclear position. We also report that the surface of the central cell nucleus is enriched in F-actin. Thus, the central cell nucleus might also serve as a site that organizes F-actin cytoskeleton to promote sperm cell nucleus movement and karyogamy.
KW - F-actin
KW - Fertilization
KW - Gamete
KW - Nuclear migration
UR - http://www.scopus.com/inward/record.url?scp=84939944925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939944925&partnerID=8YFLogxK
U2 - 10.1007/s00497-015-0259-1
DO - 10.1007/s00497-015-0259-1
M3 - Article
C2 - 25698518
AN - SCOPUS:84939944925
SN - 2194-7953
VL - 28
SP - 103
EP - 110
JO - Plant Reproduction
JF - Plant Reproduction
IS - 2
ER -