The class III peroxidase PRX17 is a direct target of the MADS-box transcription factor AGAMOUS-LIKE15 (AGL15) and participates in lignified tissue formation

Claudia Cosio, Philippe Ranocha, Edith Francoz, Vincent Burlat, Yumei Zheng, Sharyn E. Perry, Juan Jose Ripoll, Martin Yanofsky, Christophe Dunand

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Several physiological functions have been attributed to class III peroxidases (PRXs) in plants, but the in planta role of most members of this family still remains undetermined. Here, we report the first functional characterization of PRX17 (At2g22420), one of the 73 members of this family in Arabidopsis thaliana. Localization of PRX17 was examined by transient expression in Nicotiana benthamiana. Loss- and gain-of-function mutants in A. thaliana were studied. Regulation at the gene and protein levels was analyzed using β-glucuronidase (GUS) activity, quantitative reverse transcriptase (qRT)-PCR, zymography, and chromatin immunoprecipitation. Phenotypes were characterized including lignin and xylan contents. PRX17 was expressed in various tissues, including vascular tissues, and PRX17 was localized to the cell wall. In prx17, the lignin content was reduced in the stem and siliques and bolting was delayed, while the opposite phenotype was observed in 35S:PRX17 plants, together with a significant increase of lignin and xylan immunofluorescence signal. Finally, we demonstrated that the transcription factor AGAMOUS-LIKE15 (AGL15) binds to the PRX17 promoter and regulates PRX17 expression level. This converging set of structural, transcriptomic and physiological data suggests that PRX17, under the control of AGL15, contributes to developmental programs by playing an essential role in regulating age-dependent lignified tissue formation, including changes in cell wall properties.

Original languageEnglish
Pages (from-to)250-263
Number of pages14
JournalNew Phytologist
Volume213
Issue number1
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Publisher Copyright:
© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust

Keywords

  • Arabidopsis thaliana
  • chromatin immunoprecipitation-with DNA microarray (ChIP-chip)
  • development
  • flower
  • isoelectric focusing (IEF)
  • peroxidase
  • silique
  • stem

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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