The confounding effects of particle size and substrate bulk density on Phanerochaete chrysosporium pretreatment of Panicum virgatum

Amanda N. Hickman; Sue E. Nokes; William S. Sympson; Mathew J. Ruwaya; Michael Montross; Barbara L. Knutson

doi:10.15376/biores.11.3.7500-7511

The confounding effects of particle size and substrate bulk density on Phanerochaete chrysosporium pretreatment of Panicum virgatum

Amanda N. Hickman, Sue E. Nokes, William S. Sympson, Mathew J. Ruwaya, Michael Montross, Barbara L. Knutson

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Phanerochaete chrysosporium treatment is less effective as a biological pretreatment on feedstock with larger particle sizes. We hypothesized that the improved effectiveness of the pretreatment when smaller particle sizes are used may be due to the inherently higher bulk density with smaller particle sizes. The effects of substrate bulk density and particle size on the efficacy of P. chrysosporium pretreatment of switchgrass (Panicum virgatum) was tested experimentally. Phanerochaete chrysosporium was grown on senesced switchgrass (2 different particle sizes) with various bulk densities. In all treatments, the fungal-pretreated samples released more glucose during enzymatic saccharification than the control sample. Substrate bulk density was a statistically significant factor in explaining the variation in the amount of glucose released per gram of substrate used. However, the particle size was not found to be a significant factor. On-farm switchgrass pretreatment may not require particle size reduction if the switchgrass is supplied in high-density bales.

Original language	English
Pages (from-to)	7500-7511
Number of pages	12
Journal	BioResources
Volume	11
Issue number	3
DOIs	https://doi.org/10.15376/biores.11.3.7500-7511
State	Published - 2016

Bibliographical note

Funding Information:
The authors sincerely acknowledge the financial support of the United States Department of Agriculture National Institute for Food and Agriculture, Biomass Research and Development Initiative grant #2011-10006-30363. This is publication No. 16-05-030 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch-Multistate project under accession number 1003563.

Funding

The authors sincerely acknowledge the financial support of the United States Department of Agriculture National Institute for Food and Agriculture, Biomass Research and Development Initiative grant #2011-10006-30363. This is publication No. 16-05-030 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch-Multistate project under accession number 1003563.

Funders	Funder number
United States Department of Agriculture National Institute for Food and Agriculture Biomass Research and Development Initiative	16-05-030, 2011-10006-30363
U.S. Department of Agriculture	1003563
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Kentucky Agricultural Experiment Station

Keywords

Biological pretreatment
Lignocellulose
Phanerochaete chrysosporium
Switchgrass
White rot fungi

ASJC Scopus subject areas

Environmental Engineering
Bioengineering
Waste Management and Disposal

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10.15376/biores.11.3.7500-7511

Cite this

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title = "The confounding effects of particle size and substrate bulk density on Phanerochaete chrysosporium pretreatment of Panicum virgatum",

abstract = "Phanerochaete chrysosporium treatment is less effective as a biological pretreatment on feedstock with larger particle sizes. We hypothesized that the improved effectiveness of the pretreatment when smaller particle sizes are used may be due to the inherently higher bulk density with smaller particle sizes. The effects of substrate bulk density and particle size on the efficacy of P. chrysosporium pretreatment of switchgrass (Panicum virgatum) was tested experimentally. Phanerochaete chrysosporium was grown on senesced switchgrass (2 different particle sizes) with various bulk densities. In all treatments, the fungal-pretreated samples released more glucose during enzymatic saccharification than the control sample. Substrate bulk density was a statistically significant factor in explaining the variation in the amount of glucose released per gram of substrate used. However, the particle size was not found to be a significant factor. On-farm switchgrass pretreatment may not require particle size reduction if the switchgrass is supplied in high-density bales.",

keywords = "Biological pretreatment, Lignocellulose, Phanerochaete chrysosporium, Switchgrass, White rot fungi",

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note = "Funding Information: The authors sincerely acknowledge the financial support of the United States Department of Agriculture National Institute for Food and Agriculture, Biomass Research and Development Initiative grant #2011-10006-30363. This is publication No. 16-05-030 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch-Multistate project under accession number 1003563.",

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language = "English",

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AU - Ruwaya, Mathew J.

AU - Montross, Michael

AU - Knutson, Barbara L.

N1 - Funding Information: The authors sincerely acknowledge the financial support of the United States Department of Agriculture National Institute for Food and Agriculture, Biomass Research and Development Initiative grant #2011-10006-30363. This is publication No. 16-05-030 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch-Multistate project under accession number 1003563.

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N2 - Phanerochaete chrysosporium treatment is less effective as a biological pretreatment on feedstock with larger particle sizes. We hypothesized that the improved effectiveness of the pretreatment when smaller particle sizes are used may be due to the inherently higher bulk density with smaller particle sizes. The effects of substrate bulk density and particle size on the efficacy of P. chrysosporium pretreatment of switchgrass (Panicum virgatum) was tested experimentally. Phanerochaete chrysosporium was grown on senesced switchgrass (2 different particle sizes) with various bulk densities. In all treatments, the fungal-pretreated samples released more glucose during enzymatic saccharification than the control sample. Substrate bulk density was a statistically significant factor in explaining the variation in the amount of glucose released per gram of substrate used. However, the particle size was not found to be a significant factor. On-farm switchgrass pretreatment may not require particle size reduction if the switchgrass is supplied in high-density bales.

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The confounding effects of particle size and substrate bulk density on Phanerochaete chrysosporium pretreatment of Panicum virgatum

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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