The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina

Abigael J. Kosgei, Mitchell D. Miller, Minakshi Bhardwaj, Weijun Xu, Jon S. Thorson, Steven G. Van Lanen, George N. Phillips

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalActa Crystallographica Section F:Structural Biology Communications
Volume78
DOIs
StatePublished - Jan 1 2022

Bibliographical note

Publisher Copyright:
© 2022 International Union of Crystallography. All rights reserved.

Keywords

  • Anthraquinone
  • Biosynthetic gene clusters
  • Dynemicin
  • Enediynes
  • Micromonospora chersina ATCC53710
  • Natural products
  • Polyketides
  • Unknown function
  • β-barrel

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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