The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina

Abigael J. Kosgei; Mitchell D. Miller; Minakshi Bhardwaj; Weijun Xu; Jon S. Thorson; Steven G. Van Lanen; George N. Phillips

doi:10.1107/S2053230X21012322

The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina

Abigael J. Kosgei, Mitchell D. Miller, Minakshi Bhardwaj, Weijun Xu, Jon S. Thorson, Steven G. Van Lanen, George N. Phillips

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Acta Crystallographica Section F:Structural Biology Communications
Volume	78
DOIs	https://doi.org/10.1107/S2053230X21012322
State	Published - Jan 1 2022

Bibliographical note

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© 2022 International Union of Crystallography. All rights reserved.

Keywords

Anthraquinone
Biosynthetic gene clusters
Dynemicin
Enediynes
Micromonospora chersina ATCC53710
Natural products
Polyketides
Unknown function
β-barrel

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S2053230X21012322

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title = "The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina",

abstract = "Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.",

keywords = "Anthraquinone, Biosynthetic gene clusters, Dynemicin, Enediynes, Micromonospora chersina ATCC53710, Natural products, Polyketides, Unknown function, β-barrel",

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AU - Kosgei, Abigael J.

AU - Miller, Mitchell D.

AU - Bhardwaj, Minakshi

AU - Xu, Weijun

AU - Thorson, Jon S.

AU - Van Lanen, Steven G.

AU - Phillips, George N.

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

AB - Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.

KW - Anthraquinone

KW - Biosynthetic gene clusters

KW - Dynemicin

KW - Enediynes

KW - Micromonospora chersina ATCC53710

KW - Natural products

KW - Polyketides

KW - Unknown function

KW - β-barrel

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The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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