The detachment strength and morphology of bone cells contacting materials modified with a peptide sequence found within bone sialoprotein

Alireza Rezania, Carson H. Thomas, Annette B. Branger, Christopher M. Waters, Kevin E. Healy

Research output: Contribution to journalArticlepeer-review

172 Scopus citations

Abstract

Adhesion, spreading, and focal contact formation of primary bone-derived cells on quartz surfaces grafted with a 15 amino acid peptide that contained a -RGD-(-Arg-Gly-Asp-) sequence unique to bone sialoprotein was investigated. The peptide surfaces were fabricated by using a heterbifunctional crosslinker, sulfosuccinimidyal 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, to link the peptide to amine functionalized quartz surfaces. Contact angle measurements, spectroscopic ellipsometry, and X-ray photoelectron spectroscopy were used to confirm the chemistry and thickness of the overlayers. A radial flow apparatus was used to characterize cell detachment from peptide-grafted surfaces. After 20 min of cell incubation, the strength of cell adhesion was significantly (p<0.05) higher on the -RGD- compared to -RGE- (control) surfaces. Furthermore, the mean area of cells contacting the -RGD- was significantly (p<0.05) higher than -RGE- surfaces. Vinculin staining showed formation of small focal contact patches on the periphery of bone cells incubated for 2 h on the -RGD- surfaces; however, few or no focal contacts were formed by cells seeded on the -RGE-grafted surfaces. The methods of peptide immobilization utilized in this study can be applied to implants, biosensors, and diagnostic devices that require specificity in cell adhesion.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
JournalJournal of Biomedical Materials Research
Volume37
Issue number1
DOIs
StatePublished - Oct 1997

Keywords

  • Bone cells
  • Cell adhesion
  • Peptide immobilization
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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