The dose-response of canine focal gastric mucosal blood flow to misoprostol

T. J. Gana; B. R. Macpherson; J. Koo

doi:10.3109/00365528909093069

The dose-response of canine focal gastric mucosal blood flow to misoprostol

T. J. Gana, B. R. Macpherson, J. Koo

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We studied the dose-response of focal gastric mucosal blood flow measured simultaneously by laser-Doppler flowmetry and hydrogen gas clearance in the canine chambered gastric segment to topical misoprostol (0.1, 1.0, 10, 100, and 1000 μg in 10 ml of 150 mM NaCl for two 15-min periods per dose). Simultaneously obtained mucosal blood flow values showed a highly significant linear correlation (r = 0.63, n = 20, p < 0.01) in the basal but not misoprostol periods between the two techniques. Laser-Doppler flowmetry measured a dose-dependent increase in blood Row (E_max= 6.4 ± 2.8 V at the 100-mu;g dose; equivalent to 92.8% increase above the basal mean blood flow value; ED₅₀ = 1.0 μg). Peak increase in blood flow by laser-Doppler flowmetry after dosing was attained in 6.1 ± 0.7 min and maintained for 1.9 ± 0.3 min. In contrast, hydrogen gas clearance showed a gradual decline in blood flow after misoprostol administration throughout all experiments. The duration of each hydrogen gas clearance measurement was 13.1 ± 0.1 min. In conclusion, misoprostol dose-dependently and transiently increases focal gastric mucosal blood flow. However, only laser-Doppler flowmetry is sensitive enough to detect it. Although it can measure steady-state blood flow, owing to the duration of one measurement, hydrogen gas clearance is incapable of detecting rapid flow changes.

Original language	English
Pages (from-to)	423-429
Number of pages	7
Journal	Scandinavian Journal of Gastroenterology
Volume	24
Issue number	4
DOIs	https://doi.org/10.3109/00365528909093069
State	Published - 1989

Bibliographical note

Funding Information:
This study was supported in part by grant 55- 01496 to Dr. J. Koo from the Canadian Surgical Research Fund. The supply of misoprostol from G. D. Searle & Co. is highly appreciated. The authors are grateful to Dr. D. A. Cook, Dept. of Pharmacology, University of Alberta, Edmonton, for assistance with the computer program. They also thank Diane Mageau for assistance and Carolyn Ford and Colleen Gardner for secretarial assistance.

Funding

This study was supported in part by grant 55- 01496 to Dr. J. Koo from the Canadian Surgical Research Fund. The supply of misoprostol from G. D. Searle & Co. is highly appreciated. The authors are grateful to Dr. D. A. Cook, Dept. of Pharmacology, University of Alberta, Edmonton, for assistance with the computer program. They also thank Diane Mageau for assistance and Carolyn Ford and Colleen Gardner for secretarial assistance.

Funders	Funder number
Canadian Surgical Research Fund

Keywords

Blood Row
Focal
Hydrogen gas clearance
Laser-Doppler flowmetry
Mucosa
Stomach

ASJC Scopus subject areas

Gastroenterology

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10.3109/00365528909093069

Cite this

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title = "The dose-response of canine focal gastric mucosal blood flow to misoprostol",

abstract = "We studied the dose-response of focal gastric mucosal blood flow measured simultaneously by laser-Doppler flowmetry and hydrogen gas clearance in the canine chambered gastric segment to topical misoprostol (0.1, 1.0, 10, 100, and 1000 μg in 10 ml of 150 mM NaCl for two 15-min periods per dose). Simultaneously obtained mucosal blood flow values showed a highly significant linear correlation (r = 0.63, n = 20, p < 0.01) in the basal but not misoprostol periods between the two techniques. Laser-Doppler flowmetry measured a dose-dependent increase in blood Row (Emax= 6.4 ± 2.8 V at the 100-mu;g dose; equivalent to 92.8% increase above the basal mean blood flow value; ED50 = 1.0 μg). Peak increase in blood flow by laser-Doppler flowmetry after dosing was attained in 6.1 ± 0.7 min and maintained for 1.9 ± 0.3 min. In contrast, hydrogen gas clearance showed a gradual decline in blood flow after misoprostol administration throughout all experiments. The duration of each hydrogen gas clearance measurement was 13.1 ± 0.1 min. In conclusion, misoprostol dose-dependently and transiently increases focal gastric mucosal blood flow. However, only laser-Doppler flowmetry is sensitive enough to detect it. Although it can measure steady-state blood flow, owing to the duration of one measurement, hydrogen gas clearance is incapable of detecting rapid flow changes.",

keywords = "Blood Row, Focal, Hydrogen gas clearance, Laser-Doppler flowmetry, Mucosa, Stomach",

author = "Gana, {T. J.} and Macpherson, {B. R.} and J. Koo",

note = "Funding Information: This study was supported in part by grant 55- 01496 to Dr. J. Koo from the Canadian Surgical Research Fund. The supply of misoprostol from G. D. Searle & Co. is highly appreciated. The authors are grateful to Dr. D. A. Cook, Dept. of Pharmacology, University of Alberta, Edmonton, for assistance with the computer program. They also thank Diane Mageau for assistance and Carolyn Ford and Colleen Gardner for secretarial assistance.",

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AU - Gana, T. J.

AU - Macpherson, B. R.

AU - Koo, J.

N1 - Funding Information: This study was supported in part by grant 55- 01496 to Dr. J. Koo from the Canadian Surgical Research Fund. The supply of misoprostol from G. D. Searle & Co. is highly appreciated. The authors are grateful to Dr. D. A. Cook, Dept. of Pharmacology, University of Alberta, Edmonton, for assistance with the computer program. They also thank Diane Mageau for assistance and Carolyn Ford and Colleen Gardner for secretarial assistance.

PY - 1989

Y1 - 1989

N2 - We studied the dose-response of focal gastric mucosal blood flow measured simultaneously by laser-Doppler flowmetry and hydrogen gas clearance in the canine chambered gastric segment to topical misoprostol (0.1, 1.0, 10, 100, and 1000 μg in 10 ml of 150 mM NaCl for two 15-min periods per dose). Simultaneously obtained mucosal blood flow values showed a highly significant linear correlation (r = 0.63, n = 20, p < 0.01) in the basal but not misoprostol periods between the two techniques. Laser-Doppler flowmetry measured a dose-dependent increase in blood Row (Emax= 6.4 ± 2.8 V at the 100-mu;g dose; equivalent to 92.8% increase above the basal mean blood flow value; ED50 = 1.0 μg). Peak increase in blood flow by laser-Doppler flowmetry after dosing was attained in 6.1 ± 0.7 min and maintained for 1.9 ± 0.3 min. In contrast, hydrogen gas clearance showed a gradual decline in blood flow after misoprostol administration throughout all experiments. The duration of each hydrogen gas clearance measurement was 13.1 ± 0.1 min. In conclusion, misoprostol dose-dependently and transiently increases focal gastric mucosal blood flow. However, only laser-Doppler flowmetry is sensitive enough to detect it. Although it can measure steady-state blood flow, owing to the duration of one measurement, hydrogen gas clearance is incapable of detecting rapid flow changes.

AB - We studied the dose-response of focal gastric mucosal blood flow measured simultaneously by laser-Doppler flowmetry and hydrogen gas clearance in the canine chambered gastric segment to topical misoprostol (0.1, 1.0, 10, 100, and 1000 μg in 10 ml of 150 mM NaCl for two 15-min periods per dose). Simultaneously obtained mucosal blood flow values showed a highly significant linear correlation (r = 0.63, n = 20, p < 0.01) in the basal but not misoprostol periods between the two techniques. Laser-Doppler flowmetry measured a dose-dependent increase in blood Row (Emax= 6.4 ± 2.8 V at the 100-mu;g dose; equivalent to 92.8% increase above the basal mean blood flow value; ED50 = 1.0 μg). Peak increase in blood flow by laser-Doppler flowmetry after dosing was attained in 6.1 ± 0.7 min and maintained for 1.9 ± 0.3 min. In contrast, hydrogen gas clearance showed a gradual decline in blood flow after misoprostol administration throughout all experiments. The duration of each hydrogen gas clearance measurement was 13.1 ± 0.1 min. In conclusion, misoprostol dose-dependently and transiently increases focal gastric mucosal blood flow. However, only laser-Doppler flowmetry is sensitive enough to detect it. Although it can measure steady-state blood flow, owing to the duration of one measurement, hydrogen gas clearance is incapable of detecting rapid flow changes.

KW - Blood Row

KW - Focal

KW - Hydrogen gas clearance

KW - Laser-Doppler flowmetry

KW - Mucosa

KW - Stomach

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JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

IS - 4

ER -

The dose-response of canine focal gastric mucosal blood flow to misoprostol

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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