Abstract
AGAMOUS-like 15 (AGL15) is a member of the MADS domain family of transcription factors (TFs) that can directly induce and repress target gene expression, and for which promotion of somatic embryogenesis (SE) is positively correlated with accumulation. An ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif of form LxLxL within the carboxyl-terminal domain of AGL15 was shown to be involved in repression of gene expression. Here, we examine whether AGL15′ s ability to repress gene expression is needed to promote SE. While a form of AGL15 where the LxLxL is changed to AxAxA can still promote SE, another form with a strong transcriptional activator at the carboxy-terminal end, does not promote SE and, in fact, is detrimental to SE development. Select target genes were examined for response to the different forms of AGL15.
| Original language | English |
|---|---|
| Article number | 758 |
| Journal | Plants |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2021 |
Bibliographical note
Publisher Copyright:© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Funding
Funding: This work was supported by the National Science Foundation (grant no. IOS-1656380 to S.E.P.) and by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project (S.E.P.) under accession number 1013409.
| Funders | Funder number |
|---|---|
| National Science Foundation Arctic Social Science Program | IOS-1656380 |
| U.S. Department of Agriculture | 1013409 |
| US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative |
Keywords
- AGL15
- Arabidopsis thaliana
- EAR motif
- MADS-box gene
- Repression
- Seed
- Somatic embryoge-nesis
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
- Plant Science