The effect of environment on interferon-gamma production in neonatal foals

Lingshuang Sun; Amanda A. Adams; Allen Page; Alejandra Betancourt; David W. Horohov

doi:10.1016/j.vetimm.2011.06.030

The effect of environment on interferon-gamma production in neonatal foals

Lingshuang Sun, Amanda A. Adams, Allen Page, Alejandra Betancourt, David W. Horohov

Veterinary Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

While interferon-gamma (IFNγ) plays an important role in protection against viral and intracellular bacterial infections, its production in neonates is deficient. Exposure to environmental antigens can promote the maturation of the immune system of neonatal humans and mice. We hypothesize that exposure to high level of microbial components would increase the production of IFNγ in neonatal foals. To test this hypothesis, one group of foals was placed into stalls three times a week for 8 weeks. A second group of foals remained on pasture. Air samples were collected from the barn and pasture for microbial culture. There were more bacteria and fungi in the air samples collected from the barn compared with those from the pasture. Bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC) were collected from both groups of foals at various times to assess IFNγ production. The frequency of IFNγ ⁺ lymphocytes in BAL cells and PBMC was higher for foals kept in the stalls.

Original language	English
Pages (from-to)	170-175
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	143
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetimm.2011.06.030
State	Published - Sep 15 2011

Bibliographical note

Funding Information:
This work was supported by a grant from the USDA ( 2010-65119-20600 ). The anti-CD3 and CD8β antibodies were generously provided by Dr. Jeff Stott, UC Davis. The technical assistance of Eric Oberst, Nicole Combs and Daisy Ramos was appreciated. Appreciation is also extended to Lynn Ennis and the Veterinary Science Farm staff for their care of the foals.

Funding

This work was supported by a grant from the USDA ( 2010-65119-20600 ). The anti-CD3 and CD8β antibodies were generously provided by Dr. Jeff Stott, UC Davis. The technical assistance of Eric Oberst, Nicole Combs and Daisy Ramos was appreciated. Appreciation is also extended to Lynn Ennis and the Veterinary Science Farm staff for their care of the foals.

Funders	Funder number
U.S. Department of Agriculture	2010-65119-20600

Keywords

Air sample
Bronchoalveolar lavage
Environment
Interferon gamma
Lymphocyte
Neonatal foal

ASJC Scopus subject areas

Immunology
General Veterinary

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10.1016/j.vetimm.2011.06.030

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title = "The effect of environment on interferon-gamma production in neonatal foals",

abstract = "While interferon-gamma (IFNγ) plays an important role in protection against viral and intracellular bacterial infections, its production in neonates is deficient. Exposure to environmental antigens can promote the maturation of the immune system of neonatal humans and mice. We hypothesize that exposure to high level of microbial components would increase the production of IFNγ in neonatal foals. To test this hypothesis, one group of foals was placed into stalls three times a week for 8 weeks. A second group of foals remained on pasture. Air samples were collected from the barn and pasture for microbial culture. There were more bacteria and fungi in the air samples collected from the barn compared with those from the pasture. Bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC) were collected from both groups of foals at various times to assess IFNγ production. The frequency of IFNγ + lymphocytes in BAL cells and PBMC was higher for foals kept in the stalls.",

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note = "Funding Information: This work was supported by a grant from the USDA ( 2010-65119-20600 ). The anti-CD3 and CD8β antibodies were generously provided by Dr. Jeff Stott, UC Davis. The technical assistance of Eric Oberst, Nicole Combs and Daisy Ramos was appreciated. Appreciation is also extended to Lynn Ennis and the Veterinary Science Farm staff for their care of the foals. ",

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AU - Page, Allen

AU - Betancourt, Alejandra

AU - Horohov, David W.

N1 - Funding Information: This work was supported by a grant from the USDA ( 2010-65119-20600 ). The anti-CD3 and CD8β antibodies were generously provided by Dr. Jeff Stott, UC Davis. The technical assistance of Eric Oberst, Nicole Combs and Daisy Ramos was appreciated. Appreciation is also extended to Lynn Ennis and the Veterinary Science Farm staff for their care of the foals.

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N2 - While interferon-gamma (IFNγ) plays an important role in protection against viral and intracellular bacterial infections, its production in neonates is deficient. Exposure to environmental antigens can promote the maturation of the immune system of neonatal humans and mice. We hypothesize that exposure to high level of microbial components would increase the production of IFNγ in neonatal foals. To test this hypothesis, one group of foals was placed into stalls three times a week for 8 weeks. A second group of foals remained on pasture. Air samples were collected from the barn and pasture for microbial culture. There were more bacteria and fungi in the air samples collected from the barn compared with those from the pasture. Bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC) were collected from both groups of foals at various times to assess IFNγ production. The frequency of IFNγ + lymphocytes in BAL cells and PBMC was higher for foals kept in the stalls.

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The effect of environment on interferon-gamma production in neonatal foals

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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