The effect of HMGB1 on sub-toxic chlorpyrifos exposure-induced neuroinflammation in amygdala of neonatal rats

Jing Tian, Hongmei Dai, Yuanying Deng, Jie Zhang, Ying Li, Jun Zhou, Mingyi Zhao, Mengwen Zhao, Chen Zhang, Yuxi Zhang, Peipei Wang, Guoying Bing, Lingling Zhao

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Chlorpyrifos (CPF), one of organophosphorus pesticides (OPs), is associated with developmental neurotoxicity. Inflammatory response is closely related with CPF-induced neurotoxicity. The present study aimed at exploring whether sub-toxic CPF exposure on neonatal rats results in neuroinflammation that mediated by HMGB1/TLR4/NF-κB signaling pathway in the amygdala. The neonatal rats were subcutaneously injected with 5. mg/kg CPF for 4 consecutive days (postnatal day 11-14) with or without HMGB1 inhibitor, glycyrrhizin. We assessed the levels of pro-inflammatory cytokines at 12, 24, and 72. h after CPF exposure. The role of HMGB1 on neuroinflammation in sub-toxic exposure during brain development was studied. CPF-treated neonatal rats exhibited a significant increase in the expression of pro-inflammatory cytokines, such as IL-6, TNF-α and HMGB1, and a significant increase in the activation of NF-κB in the amygdala after CPF exposure. Inhibited HMGB1 reduced the release of IL-6 and TNF-α, and inhibited activation of NF-κB. Our findings indicate that CPF exposure on developmental brain might induce the activation of neuroinflammation mediated by HMGB1/TLR4/NF-κB pathway in the amygdala.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalToxicology
Volume338
DOIs
StatePublished - Dec 2 2015

Bibliographical note

Funding Information:
This research received specific grant from Key Subjects of Hunan Province (2012).

Publisher Copyright:
© 2015 Z.

Keywords

  • Chlorpyrifos
  • High-mobility group box 1
  • Inflammatory
  • Interleukin-6
  • Nuclear factor kappa B
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Toxicology

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