TY - JOUR
T1 - The effects of potassium and muscle homogenate on proprioceptive responses in crayfish and crab
AU - Malloy, Cole
AU - Dayaram, Viresh
AU - Martha, Sarah
AU - Alvarez, Brenda
AU - Chukwudolue, Ikenna
AU - Dabbain, Nadera
AU - mahmood, Dlovan D.
AU - Goleva, Slavina
AU - Hickey, Tori
AU - Ho, Angel
AU - King, Molly
AU - Kington, Paige
AU - Mattingly, Matthew
AU - Potter, Samuel
AU - Simpson, Landon
AU - Spence, Amanda
AU - Uradu, Henry
AU - Van Doorn, Jacob
AU - Weineck, Kristin
AU - Cooper, Robin L.
N1 - Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2017/7
Y1 - 2017/7
N2 - Proprioception of limbs and joints is a basic sensory function throughout most of the animal kingdom. It is important to understand how proprioceptive organs and the associated sensory neurons function with altered environments such as increased potassium ion concentrations ([K+]) from diseased states, ionic imbalances, and damaged tissues. These factors can drastically alter neuronal activity. To assess this matter, we used the chordotonal organ in a walking leg of a blue crab (Callinectes sapidus) and the muscle receptor organ of the crayfish (Procambarus clarkii). These organs serve as tractable models for the analysis of proprioception. The preparations can help serve as translational models for these effects, which may be observed in other invertebrate species as well as mammalian species (including humans). When extracellular potassium concentration ([K+]o) is increased to 20 mM in both preparations, mixed results are observed with activity increasing in some preparations and decreasing in others after mechanical displacement. However, when [K+]o is increased to 40 mM, activity drastically decreases in all preparations. Additionally, proprioceptor sensory activity declines upon exposure to a diluted muscle homogenate, which contains a host of intracellular constituents. The robust effects of altered [K+] on proprioception in these models illuminate the potential detriments on neuronal function in cases of severe tissue damage as well as altered [K+]o.
AB - Proprioception of limbs and joints is a basic sensory function throughout most of the animal kingdom. It is important to understand how proprioceptive organs and the associated sensory neurons function with altered environments such as increased potassium ion concentrations ([K+]) from diseased states, ionic imbalances, and damaged tissues. These factors can drastically alter neuronal activity. To assess this matter, we used the chordotonal organ in a walking leg of a blue crab (Callinectes sapidus) and the muscle receptor organ of the crayfish (Procambarus clarkii). These organs serve as tractable models for the analysis of proprioception. The preparations can help serve as translational models for these effects, which may be observed in other invertebrate species as well as mammalian species (including humans). When extracellular potassium concentration ([K+]o) is increased to 20 mM in both preparations, mixed results are observed with activity increasing in some preparations and decreasing in others after mechanical displacement. However, when [K+]o is increased to 40 mM, activity drastically decreases in all preparations. Additionally, proprioceptor sensory activity declines upon exposure to a diluted muscle homogenate, which contains a host of intracellular constituents. The robust effects of altered [K+] on proprioception in these models illuminate the potential detriments on neuronal function in cases of severe tissue damage as well as altered [K+]o.
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U2 - 10.1002/jez.2096
DO - 10.1002/jez.2096
M3 - Article
C2 - 29356422
AN - SCOPUS:85044404484
SN - 2471-5638
VL - 327
SP - 366
EP - 379
JO - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
JF - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
IS - 6
ER -