The effects of SNAP/SNARE complexes on the ATPase of NSF

Elena Matveeva; Sidney W. Whiteheart

doi:10.1016/S0014-5793(98)01071-0

The effects of SNAP/SNARE complexes on the ATPase of NSF

Elena Matveeva, Sidney W. Whiteheart

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The ATPase of the N-ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle-target membrane fusion. Complexes containing different combinations of NSF, α-SNAP, Vamp-2 (synaptobrevin 2), syntaxin 1, and SNAP-25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α-SNAP-containing complexes, only the α-SNAP/t-SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t-SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10-fold increase in initial hydrolysis rate. Complex containing both v- and t-SNAREs bound significantly more α-SNAP but did not stimulate the ATPase of NSF. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	211-214
Number of pages	4
Journal	FEBS Letters
Volume	435
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(98)01071-0
State	Published - Sep 18 1998

Bibliographical note

Funding Information:
We thank Dr. Susan A. Buhrow and Dr. Sergey V. Matveev for critical reading of the manuscript, Mr. Jim Smith for his excellent photographic assistance, and the members of the Whiteheart laboratory for their invaluable discussion. We would also like to thank Dr. Phyllis Hanson for the generous gift of the synaptobrevin-2 (Vamp-2) expression construct. This work was supported by the National Institutes of Health Grant HL56652 (to S.W.W.).

Keywords

AAA ATPase
Membrane fusion
N-Ethylmaleimide sensitive factor
SNAP receptor
Soluble NSF attachment protein
Vesicular traffic

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(98)01071-0

Cite this

@article{a14a8d3408dd43ca9faed06b4db00eee,

title = "The effects of SNAP/SNARE complexes on the ATPase of NSF",

abstract = "The ATPase of the N-ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle-target membrane fusion. Complexes containing different combinations of NSF, α-SNAP, Vamp-2 (synaptobrevin 2), syntaxin 1, and SNAP-25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α-SNAP-containing complexes, only the α-SNAP/t-SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t-SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10-fold increase in initial hydrolysis rate. Complex containing both v- and t-SNAREs bound significantly more α-SNAP but did not stimulate the ATPase of NSF. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "AAA ATPase, Membrane fusion, N-Ethylmaleimide sensitive factor, SNAP receptor, Soluble NSF attachment protein, Vesicular traffic",

author = "Elena Matveeva and Whiteheart, {Sidney W.}",

note = "Funding Information: We thank Dr. Susan A. Buhrow and Dr. Sergey V. Matveev for critical reading of the manuscript, Mr. Jim Smith for his excellent photographic assistance, and the members of the Whiteheart laboratory for their invaluable discussion. We would also like to thank Dr. Phyllis Hanson for the generous gift of the synaptobrevin-2 (Vamp-2) expression construct. This work was supported by the National Institutes of Health Grant HL56652 (to S.W.W.).",

year = "1998",

month = sep,

day = "18",

doi = "10.1016/S0014-5793(98)01071-0",

language = "English",

volume = "435",

pages = "211--214",

number = "2-3",

}

TY - JOUR

T1 - The effects of SNAP/SNARE complexes on the ATPase of NSF

AU - Matveeva, Elena

AU - Whiteheart, Sidney W.

N1 - Funding Information: We thank Dr. Susan A. Buhrow and Dr. Sergey V. Matveev for critical reading of the manuscript, Mr. Jim Smith for his excellent photographic assistance, and the members of the Whiteheart laboratory for their invaluable discussion. We would also like to thank Dr. Phyllis Hanson for the generous gift of the synaptobrevin-2 (Vamp-2) expression construct. This work was supported by the National Institutes of Health Grant HL56652 (to S.W.W.).

PY - 1998/9/18

Y1 - 1998/9/18

N2 - The ATPase of the N-ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle-target membrane fusion. Complexes containing different combinations of NSF, α-SNAP, Vamp-2 (synaptobrevin 2), syntaxin 1, and SNAP-25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α-SNAP-containing complexes, only the α-SNAP/t-SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t-SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10-fold increase in initial hydrolysis rate. Complex containing both v- and t-SNAREs bound significantly more α-SNAP but did not stimulate the ATPase of NSF. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - The ATPase of the N-ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle-target membrane fusion. Complexes containing different combinations of NSF, α-SNAP, Vamp-2 (synaptobrevin 2), syntaxin 1, and SNAP-25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α-SNAP-containing complexes, only the α-SNAP/t-SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t-SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10-fold increase in initial hydrolysis rate. Complex containing both v- and t-SNAREs bound significantly more α-SNAP but did not stimulate the ATPase of NSF. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - AAA ATPase

KW - Membrane fusion

KW - N-Ethylmaleimide sensitive factor

KW - SNAP receptor

KW - Soluble NSF attachment protein

KW - Vesicular traffic

UR - http://www.scopus.com/inward/record.url?scp=0032544437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032544437&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(98)01071-0

DO - 10.1016/S0014-5793(98)01071-0

M3 - Article

C2 - 9762911

AN - SCOPUS:0032544437

SN - 0014-5793

VL - 435

SP - 211

EP - 214

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

The effects of SNAP/SNARE complexes on the ATPase of NSF

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this