The effects of the Bowman-Birk protease inhibitor on c-myc expression and cell proliferation in the unirradiated and irradiated mouse colon

W. H.St Clair, P. C. Billings, A. R. Kennedy

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The levels of c-myc RNA and crypt cell proliferation were monitored in the mouse colonic mucosa following X-irradiation with and without oral administration of the Bowman-Birk protease inhibitor (BBI). Mice were divided into 4 groups and treated as follows: (A) daily gavage with water; (B) daily gavage with BBI; (C) daily gavage with water and 12 Gy of abdominal irradiation 1 day after the first gavage; (D) daily gavage with BBI and 12 Gy of abdominal irradiation 1 day after the first gavage. Samples were collected at various times after X-irradiation and ascending colon samples were taken for crypt cell proliferation analysis. The mucosa was removed from the remaining sample and total cellular RNA was isolated. The levels of c-myc mRNA underwent a time-dependent change following X-irradiation. Expression of the c-myc gene was unchanged at 1 day and 3 weeks after irradiation, but was markedly elevated at 1 week after X-irradiation. BBI was found to suppress the radiation induced elevation of c-myc mRNA, while having no effect on the rate of crypt cell proliferation or body weight of the mice.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalCancer Letters
Volume52
Issue number2
DOIs
StatePublished - Jul 16 1990

Bibliographical note

Funding Information:
This investigatiown ass upportedb y NIH grants CA 38246, CA 46496, RR-05404 and NIH trainingG rantC A 09078.

Keywords

  • c-myc
  • cell proliferation
  • inhibitor
  • mouse colon

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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