The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila

Catherine E. Stanley; Rebecca Adams; Jeremy Nadolski; Ellora Amrit; Matthew Barrett; Catherine Bohnett; Kelsey Campbell; Keegan Deweese; Sabbyasachi Dhar; Barbara Gillis; Carson Hill; Morgan Inks; Katrina Kozak; Alexa Larson; Ibraheem Murtaza; Destaneh Nichols; Rafael Roberts; Hannah Tyger; Courtney Waterbury; Robin L. Cooper

doi:10.1007/s10158-020-00243-5

The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila

Catherine E. Stanley, Rebecca Adams, Jeremy Nadolski, Ellora Amrit, Matthew Barrett, Catherine Bohnett, Kelsey Campbell, Keegan Deweese, Sabbyasachi Dhar, Barbara Gillis, Carson Hill, Morgan Inks, Katrina Kozak, Alexa Larson, Ibraheem Murtaza, Destaneh Nichols, Rafael Roberts, Hannah Tyger, Courtney Waterbury, Robin L. Cooper

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Abstract: Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. Graphic abstract: [Figure not available: see fulltext.]

Original language	English
Article number	10
Journal	Invertebrate Neuroscience
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/s10158-020-00243-5
State	Published - Sep 1 2020

Bibliographical note

Funding Information:
Funded by Dept. of Biology, Univ. of KY. and by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103436 (R.A.)

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Anesthetics
Cardiac
Crustacean
Insect
Invertebrate
MS-222
Neuromuscular
Sensory

ASJC Scopus subject areas

Developmental Neuroscience
Cellular and Molecular Neuroscience

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Stanley, C. E., Adams, R., Nadolski, J., Amrit, E., Barrett, M., Bohnett, C., Campbell, K., Deweese, K., Dhar, S., Gillis, B., Hill, C., Inks, M., Kozak, K., Larson, A., Murtaza, I., Nichols, D., Roberts, R., Tyger, H., Waterbury, C., & Cooper, R. L. (2020). The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila. Invertebrate Neuroscience, 20(3), [10]. https://doi.org/10.1007/s10158-020-00243-5

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title = "The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila",

abstract = "Abstract: Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. Graphic abstract: [Figure not available: see fulltext.]",

keywords = "Anesthetics, Cardiac, Crustacean, Insect, Invertebrate, MS-222, Neuromuscular, Sensory",

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note = "Funding Information: Funded by Dept. of Biology, Univ. of KY. and by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103436 (R.A.) Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",

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Stanley, CE, Adams, R, Nadolski, J, Amrit, E, Barrett, M, Bohnett, C, Campbell, K, Deweese, K, Dhar, S, Gillis, B, Hill, C, Inks, M, Kozak, K, Larson, A, Murtaza, I, Nichols, D, Roberts, R, Tyger, H, Waterbury, C & Cooper, RL 2020, 'The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila', Invertebrate Neuroscience, vol. 20, no. 3, 10. https://doi.org/10.1007/s10158-020-00243-5

TY - JOUR

T1 - The effects of tricaine mesylate on arthropods

T2 - crayfish, crab and Drosophila

AU - Stanley, Catherine E.

AU - Adams, Rebecca

AU - Nadolski, Jeremy

AU - Amrit, Ellora

AU - Barrett, Matthew

AU - Bohnett, Catherine

AU - Campbell, Kelsey

AU - Deweese, Keegan

AU - Dhar, Sabbyasachi

AU - Gillis, Barbara

AU - Hill, Carson

AU - Inks, Morgan

AU - Kozak, Katrina

AU - Larson, Alexa

AU - Murtaza, Ibraheem

AU - Nichols, Destaneh

AU - Roberts, Rafael

AU - Tyger, Hannah

AU - Waterbury, Courtney

AU - Cooper, Robin L.

N1 - Funding Information: Funded by Dept. of Biology, Univ. of KY. and by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103436 (R.A.) Publisher Copyright: © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Abstract: Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. Graphic abstract: [Figure not available: see fulltext.]

AB - Abstract: Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. Graphic abstract: [Figure not available: see fulltext.]

KW - Anesthetics

KW - Cardiac

KW - Crustacean

KW - Insect

KW - Invertebrate

KW - MS-222

KW - Neuromuscular

KW - Sensory

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M1 - 10

ER -

The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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