The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila

Catherine E. Stanley, Rebecca Adams, Jeremy Nadolski, Ellora Amrit, Matthew Barrett, Catherine Bohnett, Kelsey Campbell, Keegan Deweese, Sabbyasachi Dhar, Barbara Gillis, Carson Hill, Morgan Inks, Katrina Kozak, Alexa Larson, Ibraheem Murtaza, Destaneh Nichols, Rafael Roberts, Hannah Tyger, Courtney Waterbury, Robin L. Cooper

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Abstract: Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. Graphic abstract: [Figure not available: see fulltext.]

Original languageEnglish
Article number10
JournalInvertebrate Neuroscience
Issue number3
StatePublished - Sep 1 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Anesthetics
  • Cardiac
  • Crustacean
  • Insect
  • Invertebrate
  • MS-222
  • Neuromuscular
  • Sensory

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience


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