The electrospray ionization - Mass spectra of erythromycin A obtained from a marine Streptomyces sp. mutant

A. El-Bondkly, Howaida Abd-Alla, M. Shaaban, K. Shaaban

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In our ongoing search for production improvements of bioactive secondary metabolites from marine Streptomyces through the induction of mutations using UV light, out of 145 isolates, mutant 10/14 was able to produce potent antibacterial metabolites other than the parent strain as established by chromatographic analysis. Up-scaling fermentation of mutant 10/14, followed by working up and isolation delivered five metabolites, phenazine, 1-acetyl-β-carboline, perlolyrin and erythromycin A, along with an oily substance. The latter two compounds were responsible for the antibacterial activity of the strain. In this article, we discuss with the mutation of the marine Streptomyces sp. AH2, bioactivity evaluation, fermentation and isolation of the microbial metabolites. Moreover, we study to first time in detail the 1D and 2D NMR and ESI MS data including ESI MS2 and MS3 patterns combined with HRESI MS of erythromycin A.

Original languageEnglish
Pages (from-to)310-319
Number of pages10
JournalIndian Journal of Pharmaceutical Sciences
Volume70
Issue number3
DOIs
StatePublished - Feb 1 2008

Keywords

  • Bioactivity evaluation
  • ESI-MS/MS
  • Erythromycin A
  • Marine Streptomyces mutation

ASJC Scopus subject areas

  • Pharmaceutical Science

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